Figure 4From: Glucose induces rapid changes in the secretome of Saccharomyces cerevisiaeGlucose is required for the decrease of gluconeogenic enzymes in the extracellular fraction. (A) Wild-type cells expressing either Fbp1p-GFP, Mdh2p-GFP, Icl1p-GFP, Pck1p-GFP, or Mls1p-GFP were grown in YPKG media for 3d. Cells were harvested or transferred to YPD media for 0, 15, and 30Â minutes and harvested. The distribution of GFP tagged proteins in the intracellular (I) and extracellular (E) fractions were determined using Western blotting with anti-GFP antibodies. (B), Wild-type cells expressing either Fbp1p-GFP, Mdh2p-GFP, Icl1p-GFP, Pck1p-GFP, or Mls1p-GFP were transferred from YPKG to YP media containing 0% added glucose for 0, 15, and 30Â minutes. Levels of GFP tagged proteins in the intracellular (I) and extracellular (E) fractions were determined using Western blotting with anti-GFP antibodies.Back to article page