Figure 8

Transferring cells from YPKG to YPD causes a rapid disappearance of small vesicles from the extracellular fraction. (A and B) Wild-type cells were grown in YPKG or transferred to YPD for 30 minutes and extracted. Total extracts from t = 0 (A) and t = 30 (B) cells were stained with uranyl acetate and visualized by transmission electron microscope as described in Methods. Bars: 200 nm. (C) Quantification of the number of 30–50 nm small vesicles per μm² in total extracts in t = 0 and t = 30 cells. Mean and SD were derived from counting the number of small vesicles using 3 images taken from extracts isolated from t = 0 and t = 30 cells. (D) Quantification of the number of 100–300 nm structures per μm² in total extracts in t = 0 and t = 30 cells. Mean and SD were obtained from counting the number of 100–300 nm structures from 3 images taken from total extracts isolated from t = 0 and t = 30 cells.