Table 1 Comparison of functions of proteins identified in iTRAQ1 and iTRAQ2 to the yeast ORFs
From: Glucose induces rapid changes in the secretome of Saccharomyces cerevisiae
Go functions | A | B | C | |||
|---|---|---|---|---|---|---|
|  | iTRAQ1 (n = 264) | iTRAQ2 (n = 324) | Genome (n = 6334) | |||
| Â | Frequency | % | Frequency | % | Frequency | % |
Biosynthetic process | 135 | 50.90% | 166 | 47.60% | 2066 | 32.60% |
Small molecule metabolic process | 107 | 40.40% | 133 | 38.10% | 661 | 10.40% |
Cellular nitrogen compound metabolic process | 101 | 38.10% | 126 | 36.10% | 1847 | 29.20% |
Catabolic process | 54 | 20.40% | 70 | 20.10% | 663 | 10.50% |
Response to stress | 52 | 19.60% | 67 | 19.20% | 642 | 10.10% |
Cellular amino acid metabolic process | 51 | 19.20% | 64 | 18.30% | 247 | 3.90% |
Carbohydrate metabolic process | 48 | 18.10% | 54 | 15.50% | 268 | 4.20% |
Transport | 43 | 16.20% | 54 | 15.50% | 1084 | 17.10% |
Translation | 39 | 14.70% | 45 | 12.90% | 703 | 11.10% |
Generation of precursor metabolites and energy | 31 | 11.70% | 35 | 10.00% | 161 | 2.50% |
Cofactor metabolic process | 19 | 7.20% | 24 | 6.90% | 146 | 2.30% |
Protein folding | 17 | 6.40% | 23 | 6.60% | 89 | 1.40% |
Cellular component assembly | 17 | 6.40% | 21 | 6.00% | 598 | 9.40% |
Cell cycle | 17 | 6.40% | 20 | 5.70% | 531 | 6.30% |
Homeostatic process | 17 | 6.40% | 20 | 5.70% | 225 | 3.60% |
Lipid metabolic process | 17 | 6.40% | 22 | 6.30% | 295 | 8.40% |
Cellular protein modification process | 16 | 6.00% | 21 | 6.00% | 612 | 4.70% |
Ribosome biogenesis | 15 | 5.70% | 18 | 5.20% | 402 | 6.30% |
Reproduction | 15 | 5.70% | 18 | 5.20% | 429 | 6.80% |
DNA metabolic process | 14 | 5.30% | 14 | 4.00% | 455 | 7.20% |
Biological process unknown | 14 | 5.30% | 32 | 9.20% | 1188 | 18.80% |
Transcription, DNA-dependent | 13 | 4.90% | 15 | 4.30% | 640 | 10.10% |
Sulfur compound metabolic process | 13 | 4.90% | 14 | 4.00% | 89 | 1.40% |
Membrane organization | 12 | 4.50% | 16 | 4.60% | 214 | 3.40% |
Chromosome organization | 12 | 4.50% | 15 | 4.30% | 410 | 6.50% |
Vesicle-mediated transport | 12 | 4.50% | 14 | 4.00% | 346 | 5.50% |
Cell wall organization or biogenesis | 11 | 4.20% | 13 | 3.70% | 186 | 2.90% |
Aging | 11 | 4.20% | 12 | 3.40% | 70 | 1.10% |
Cytoskeleton organization | 10 | 3.80% | 16 | 4.60% | 220 | 3.50% |
tRNA metabolic process | 10 | 3.80% | 14 | 4.00% | 158 | 2.50% |
Macromolecular complex assembly | 10 | 3.80% | 12 | 3.40% | 421 | 6.60% |
Protein targeting | 10 | 3.80% | 17 | 4.90% | 262 | 4.10% |
Cell differentiation | 10 | 3.80% | 11 | 3.20% | 154 | 2.40% |
Anatomical structure development | 10 | 3.80% | 11 | 3.20% | 150 | 2.40% |
Anatomical structure formation in morphogenesis | 10 | 3.80% | 11 | 3.20% | 129 | 2.00% |
Mitochondrion organization | 9 | 3.40% | 10 | 2.90% | 339 | 5.40% |
Nucleocytoplasmic transport | 9 | 3.40% | 8 | 2.30% | 162 | 2.60% |
Ribonucleoprotein complex assembly | 8 | 3.00% | 9 | 2.60% | 130 | 2.10% |
Signal transduction | 8 | 3.00% | 10 | 2.90% | 162 | 3.50% |
Growth | 7 | 2.60% | 8 | 2.30% | 142 | 2.20% |
Transmembrane transport | 7 | 2.60% | 8 | 2.30% | 220 | 3.50% |
Nucleobase-containing compound catabolic process | 6 | 2.30% | 8 | 2.30% | 158 | 2.50% |
Mitosis | 5 | 1.90% | 6 | 1.70% | 119 | 1.90% |
Chromosome segregation | 5 | 1.90% | 6 | 1.70% | 132 | 2.10% |
Cell death | 4 | 1.50% | 5 | 1.40% | 25 | 0.40% |
Cell division | 4 | 1.50% | 6 | 1.70% | 134 | 2.10% |
Protein complex assembly | 3 | 1.10% | 4 | 1.10% | 221 | 3.50% |
mRNA processing | 2 | 0.80% | 2 | 0.60% | 192 | 3.00% |
Cytoskeleton-dependent intracellular transport | 2 | 0.80% | 2 | 0.60% | 19 | 0.30% |
Cell adhesion | 1 | 0.40% | 1 | 0.30% | 10 | 0.20% |
Plasma membrane organization | 1 | 0.40% | 1 | 0.30% | 6 | 0.10% |
Vacuolar transport | 1 | 0.40% | 1 | 0.30% | 142 | 2.20% |
Cell morphogenesis | 1 | 0.40% | 1 | 0.30% | 29 | 0.50% |
Protein maturation | 0 | 0.00% | 0 | 0.00% | 45 | 0.70% |