Cell cycle dependent association of replication licensing complex with chromatin. Proteins eluted from chromatin assembled for 30 min in Xenopus egg extracts were separated on pH 3–10 2D gels and stained with SYPRO Ruby. In control assembly reaction MCM2,3,4,6 and 7, were detectable in eluates as multiple spot chains (A). In the presence of geminin, association of MCMs with chromatin was prevented (B). The treatment of control chromatin eluates with λ-phosphatase changes the MCMs presentation in the 2D gel (C).