Table 1 Set up of the Taguchi optimisation experiment presented in Figure 2 with total number of spots detected in each gel.
From: Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach
Buffer* | Ampholytes(%) | CHAPS(%) | ASB14(%) | DTT(mM) | Spot number** |
|---|---|---|---|---|---|
1 | 0.5 | 0.5 | 0.4 | 20 | 361 |
2 | 0.5 | 1.0 | 0.8 | 40 | 339 |
3 | 0.5 | 2.0 | 1.6 | 80 | 339 |
4 | 1.0 | 0.5 | 0.8 | 80 | 296 |
5 | 1.0 | 1.0 | 1.6 | 20 | 351 |
6 | 1.0 | 2.0 | 0.4 | 40 | 355 |
7 | 2.0 | 0.5 | 1.6 | 40 | 319 |
8 | 2.0 | 1.0 | 0.4 | 80 | 327 |
9 | 2.0 | 2.0 | 0.8 | 20 | 299 |