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Table 1 Set up of the Taguchi optimisation experiment presented in Figure 2 with total number of spots detected in each gel.

From: Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach

Buffer* Ampholytes(%) CHAPS(%) ASB14(%) DTT(mM) Spot number**
1 0.5 0.5 0.4 20 361
2 0.5 1.0 0.8 40 339
3 0.5 2.0 1.6 80 339
4 1.0 0.5 0.8 80 296
5 1.0 1.0 1.6 20 351
6 1.0 2.0 0.4 40 355
7 2.0 0.5 1.6 40 319
8 2.0 1.0 0.4 80 327
9 2.0 2.0 0.8 20 299
  1. * – all buffers contained 7 M urea, 2 M thiourea, 10 mM Tris
  2. ** – number of spots detected, using Phoretix 2D Pro imaging software
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