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Table 10 Array 6: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature

B

W

A

W+A

LLQ (pg/mL)

ULQ (pg/mL)

6Ckine

0

100

0

100

152

27541

ACE

0

60

40

100

3283

93777

CA125

19

81

1

81

273

120834

CNTF

67

33

0

33

2562

109254

ET-3

9

83

8

91

881

25735

Endostatin

0

17

83

100

432

9966

ErbB1

1

95

4

99

3864

86553

ErbB2

12

88

0

88

2293

118586

FGF R3 (IIIb)

47

53

0

53

455

89028

FGF R3 (IIIc)

53

47

0

47

214

51823

FGF-6

42

58

0

58

220

18535

G-CSF

69

31

0

31

1487

49342

HB-EGF

41

59

0

59

47

3143

IFN-a

41

59

0

59

20

5021

LIF

52

48

0

48

655

52660

MMP-1

3

97

0

97

537

103951

MMP-2

0

99

1

100

1446

154542

OPN

0

97

3

100

496

60615

PAI-1

0

10

90

100

22

8289

PDGF Rb

14

86

0

86

645

59309

PEDF

0

19

81

100

2599

63199

TGF-b RIII

10

50

40

90

595

13518

Tie-2

43

57

0

57

6005

147662

VEGF R3

10

90

0

90

466

37150

uPA

12

88

0

88

89

16374

uPAR

11

89

0

89

1259

125972

VCAM-1

0

49

51

100

1577

150401

  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.