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Table 10 Array 6: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature B W A W+A LLQ (pg/mL) ULQ (pg/mL)
6Ckine 0 100 0 100 152 27541
ACE 0 60 40 100 3283 93777
CA125 19 81 1 81 273 120834
CNTF 67 33 0 33 2562 109254
ET-3 9 83 8 91 881 25735
Endostatin 0 17 83 100 432 9966
ErbB1 1 95 4 99 3864 86553
ErbB2 12 88 0 88 2293 118586
FGF R3 (IIIb) 47 53 0 53 455 89028
FGF R3 (IIIc) 53 47 0 47 214 51823
FGF-6 42 58 0 58 220 18535
G-CSF 69 31 0 31 1487 49342
HB-EGF 41 59 0 59 47 3143
IFN-a 41 59 0 59 20 5021
LIF 52 48 0 48 655 52660
MMP-1 3 97 0 97 537 103951
MMP-2 0 99 1 100 1446 154542
OPN 0 97 3 100 496 60615
PAI-1 0 10 90 100 22 8289
PDGF Rb 14 86 0 86 645 59309
PEDF 0 19 81 100 2599 63199
TGF-b RIII 10 50 40 90 595 13518
Tie-2 43 57 0 57 6005 147662
VEGF R3 10 90 0 90 466 37150
uPA 12 88 0 88 89 16374
uPAR 11 89 0 89 1259 125972
VCAM-1 0 49 51 100 1577 150401
  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.