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Table 8 Array 4: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature B W A W+A LLQ (pg/mL) ULQ (pg/mL)
ALCAM 0 100 0 100 991 145528
CD27 10 90 0 90 508 148468
CD30 53 47 0 47 2460 128280
CTACK 0 100 0 100 43 10691
Eot-3 37 63 0 63 130 28149
FGF-2 31 67 2 69 102 5814
FGF-4 57 43 0 43 260 14563
Follistatin 8 92 0 92 138 57120
GRO-g 12 75 13 88 59 4344
I-TAC 11 89 0 89 16 6191
ICAM-1 1 76 23 99 289 29018
IFN-g 39 60 1 61 14 7365
IFN-w 37 58 5 63 1177 42508
IGF-II 0 85 15 100 46 13538
IGF-1R 33 67 0 67 330 91601
IGFBP-1 2 72 26 98 272 85578
IGFBP-3 0 7 93 100 5530 30760
IGFBP-4 0 80 20 100 410 22880
IL-1 sR1 30 70 0 70 534 54857
IL-10rb 12 88 0 88 28 11331
IL-16 28 72 0 72 724 86874
IL-1 srII 19 81 0 81 509 76440
IL-2rb 71 29 0 29 10277 107828
LT bR 14 86 0 86 34 37957
Lymphotactin 28 72 0 72 166 9216
M-CSF R 0 91 9 100 1951 121318
MIP-3a 16 84 0 84 13 3389
MMP-10 10 90 0 90 158 42033
PDGF-Ra 30 66 5 70 8112 151722
PF4 0 23 77 100 67 5458
TGF-a 33 67 0 67 35 2678
TIMP-2 0 12 88 100 130 25224
TRAIL R1 33 67 0 67 21 10956
VAP-1 1 23 76 99 4624 150226
VE-cadherin 1 94 5 99 2652 146826
VEGF-D 34 66 0 66 1370 100539
b-NGF 27 73 0 73 141 12400
  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.