Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Table 9 Array 5: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

Feature	B	W	A	W+A	LLQ (pg/mL)	ULQ (pg/mL)
4-1BB	46	54	0	54	334	92233
ACE-2	37	63	0	63	1138	128330
AFP	4	96	0	96	17	11483
AgRP	18	82	0	82	72	13198
CD141	0	62	38	100	780	14852
CD40	30	70	0	70	101	19145
CNTF Ra	18	82	0	82	46	21212
CRP	4	28	68	96	201	12376
D-Dimer DD5	0	4	96	100	12070	65452
E-Selectin	0	85	15	100	89	21531
HCG	40	59	0	60	345	18736
IGFBP-6	0	2	98	100	855	38589
IL-12p40	44	56	0	56	2505	159213
IL-18	0	100	0	100	5	3743
LIF Ra	45	54	1	55	5350	117637
MIF	3	84	13	97	9753	132966
MMP-8	0	82	18	100	111	48374
NAP-2	0	19	81	100	103	9114
Neut Elast	0	66	34	100	376	24009
P-Selectin	0	82	18	100	2128	93967
PAI-II	28	72	0	72	251	115374
Prolactin	0	96	4	100	1133	77120
Protein C	0	83	17	100	1266	154054
Protein S	0	1	99	100	10808	64646
TSH	43	57	0	57	81	15614

Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.

ISSN: 1477-5956