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Table 9 Array 5: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature B W A W+A LLQ (pg/mL) ULQ (pg/mL)
4-1BB 46 54 0 54 334 92233
ACE-2 37 63 0 63 1138 128330
AFP 4 96 0 96 17 11483
AgRP 18 82 0 82 72 13198
CD141 0 62 38 100 780 14852
CD40 30 70 0 70 101 19145
CNTF Ra 18 82 0 82 46 21212
CRP 4 28 68 96 201 12376
D-Dimer DD5 0 4 96 100 12070 65452
E-Selectin 0 85 15 100 89 21531
HCG 40 59 0 60 345 18736
IGFBP-6 0 2 98 100 855 38589
IL-12p40 44 56 0 56 2505 159213
IL-18 0 100 0 100 5 3743
LIF Ra 45 54 1 55 5350 117637
MIF 3 84 13 97 9753 132966
MMP-8 0 82 18 100 111 48374
NAP-2 0 19 81 100 103 9114
Neut Elast 0 66 34 100 376 24009
P-Selectin 0 82 18 100 2128 93967
PAI-II 28 72 0 72 251 115374
Prolactin 0 96 4 100 1133 77120
Protein C 0 83 17 100 1266 154054
Protein S 0 1 99 100 10808 64646
TSH 43 57 0 57 81 15614
  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.