Figure 1

Native binding assay between Apaf-1 and Caspase-9 detected by PAGE. Binding reactions were performed using an equal amount of putative interacting histidine-tagged native recombinant proteins and incubation overnight in the binding buffer containing 50 mM Tris, 100 mM NaCl, pH 8.0. The binding was analyzed by a continuous Poly-Acrylamide Gel Electrophoresis (PAGE) (10%) followed by Coomassie Blue staining.