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Figure 3 | Proteome Science

Figure 3

From: Target selection of soluble protein complexes for structural proteomics studies

Figure 3

Immunoprecipitation of putative CARD binary complexes obtained from co-expression assay. Protein lysates obtained from the co-expressed E. coli putative CARD complexes were immunoprecipitated with anti-6XHis or anti-GST antibody. The precipitated protein samples (lane 3) were loaded onto a SDS-PAGE along with the individually-expressed protein lysates (lane 1 and 3). The protein samples from the gel were transferred onto Hybond-ECL (Amersham Biosciences). The immunoprecipitated protein complexes were detected with anti-GST and anti-6XHis antibody.

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