Figure 4
From: Target selection of soluble protein complexes for structural proteomics studies
Binary complex formation of CARD protein complex in rabbit reticulocyte lysates. Equal amounts of in vitro expressed proteins were incubated in rabbit reticulocyte lysates at 4°C for 1 hour. After incubation, protein and complexes were immunoprecipitated with anti-6XHis antibody. The precipitated samples (lane 2) together with the individually-expressed proteins (lane 1 and 3) were detected by western blot using anti-GST antibody.