SDS
|
+++
|
-
|
IEF incompatible
|
CHAPS
|
++
|
+++
|
Poor extraction of hydrophobic and high molecular weight proteins
|
trans, trans-farnesol
|
+++
|
++
|
Natural isoprenoid
|
MEGA-8a
|
+++
|
++
|
Group of nonionic detergents commonly used for protein purification [35,36]
|
MEGA-9b
|
+++
|
++
| |
MEGA-10c
|
+++
|
+++
| |
amidosulfobetaine-14 (ASB-14)
|
+++
|
++
|
Sulfobetaine-based detergents reported to improve membrane protein extraction [24-26]
|
Zwittergent® 3–10/SB 3–10d
|
+++
|
+++
| |
LPC (synthetic, lauroyl chain)e
|
+++
|
+++
|
Zwitterionic lysophospholipid
|
LPC (egg, mixed chain)e
|
++
|
++
|
Zwitterionic lysophospholipid
|
LPS (bovine brain)f
|
++
|
-
|
Anionic lysophospholipid, incompatible with IEF
|
LPE (egg, mixed chain)g
|
-
|
-
|
Zwitterionic lysophospholipid; low solubility in high urea buffer
|
LPG (egg, mixed chain)h
|
++
|
-
|
Anionic lysophospholipid, incompatible with IEF
|
LPA (egg, mixed chain)i
|
++
|
-
|
Anionic lysophospholipid, incompatible with IEF
|
cardiolipin (bovine heart)
|
++
|
-
|
Anionic lipid, incompatible with IEF, low solubility in high urea buffer
|
5,7-docosadiynoic acid
|
-
|
-
|
Synthetic fatty acid; low solubility in high urea buffer
|
lauric acid
|
+++
|
++
|
Medium chain fatty acid; low solubility in high urea buffer
|
free fatty acids (mixed)
|
-
|
-
|
Mixed natural fatty acids; low solubility in high urea buffer
|
DODAPj
|
+++
|
-
|
Cationic lipid used as a transfection reagent [37], IEF incompatible
|
1-oleoyl-sn-glycerol
|
+
|
-
|
Uncharged monoacylated lipid, very low solubility in high urea buffer
|
C12E8
k
|
+
|
-
|
Nonionic detergent used to study membrane proteins [38]
|
DL-α-O-benzylglycerol
|
+
|
-
|
Amphipathic cyclic glycerol conjugate
|
tryptophol
|
++
|
-
|
Amphipathic heterocyclic metabolite of tryptophan; ionizable at low pH
|