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Figure 1 | Proteome Science

Figure 1

From: Cognate peptide-receptor ligand mapping by directed phage display

Figure 1

Insert modification of pORFES to pORFES IV to permit cloningof blunt-ended fragments. The original pORFES [12, 14] vector was designed to accept inserts as NheI-HindIII fragments. To create pORFES IV, the NheIHindIII stuffer was replaced with aninsert to include a NaeI site, an additional bp and a (Gly)4 spacer. The ligation of inserts restoring the correct frame of the β-lactamase confers carbenicillin resistance.

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