Figure 3From: High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcellDiameter of cleared zone plotted as a function of increasing concentration of an individual improved CelF mutant. One, 3.0, 5.0 and 10.0 μL of a 27.7 ng/μL solution of F9MP mutant (27.7, 83.1, 139 and 277 ng, respectively) were spotted on an azo-CMC plate at pH 5.8. The resultant cleared zones are pictured in the inset above the graph (incubation at 10 hours, 37°C).Back to article page