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Figure 6 | Proteome Science

Figure 6

From: High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell

Figure 6

Azo-CMC plate analysis of expressed proteins from manually picked (MP) individual colonies. Individual mutants are identified by row (letter) and column (number) on the plate and by method of picking. Columns 1–3 on each plate were picked from colonies corresponding to multiplexed well B11 (see Figure 2) (high activity variant F2MP is in row F and column 2), columns 4–6 from C12 (high activity variants are E5MP and F5MP), columns 7–9 from G2 (E7MP and F9MP), columns 10–12 from G11 (F12MP). Note that F9MP has the most exceptional activity. Replicates of F9MP activity at pH 4.0 (incubated for 24 hours) are enlarged to better visualize cleared zones. Boxed mutants were selected for Western blot analysis (Figure 8) based on activity and stability

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ISSN: 1477-5956