Skip to main content

Table 1 Table 1

From: High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell

Clone Plate Sequence of CelF Terminal Codons pH Range 50°C, 1 Hour Stability
Mutagenesis    NNR NNR NNR NNR *   
WT #5 CONTROL From single 96-clone screen AAT GCT AGA CCA GGA TTC TAA 5.0–5.8 Moderate
    Asn Ala Arg Pro Gly Phe *   
#62 CONTROL From single 96-clone screen AAT GCT CCA GTA CGG GAA TAA 5.0–5.8 Moderate
    Asn Ala Pro Val Arg Glu *   
COLONY PICKING   Variants with High Activity at Low pH   
MAN AUTO           
E7   From G2 well of 768-clone multiplex screen AAT GCT TTA TAA     4.0–5.8 Not Stable
    Asn Ala Leu *      
F2 G12 From B11 well of 768-clone multiplex screen AAT GCT CAA TGG ACA CCA TAA 4.0–5.8 Moderate
    Asn Ala Gln Trp Thr Pro *   
F9 φ G4 φ From G2 well of 768-clone multiplex screen AAT GCT ATG CCA ACG TTA TAA 4.0–5.8 Very Stable
    Asn Ala Met Pro Thr Leu *   
F12   From G11 well of 768-clone multiplex screen AAT GCT CCA TAA     4.0–5.8 Moderate
    Asn Ala Pro *      
HTS Mutagenesis    NNR/Y NNR/Y NNR/Y NNR/Y *   
  C7 From Plate 2 well of 23,424- clone multiplex screen AAT GCT GGG TTA GAA TAG TAA 4.0–5.8 Very Stable
    Asn Ala Gly Leu Glu * *   
  1. φ An additional Thr to Ser mutation at amino acid position 213 in F9 and G4 clones