Clone | Plate | Sequence of CelF Terminal Codons | pH Range | 50°C, 1 Hour Stability | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Mutagenesis | Â | Â | NNR | NNR | NNR | NNR | * | Â | Â | ||
WT #5 CONTROL | From single 96-clone screen | AAT | GCT | AGA | CCA | GGA | TTC | TAA | 5.0–5.8 | Moderate | |
 |  |  | Asn | Ala | Arg | Pro | Gly | Phe | * |  |  |
#62 CONTROL | From single 96-clone screen | AAT | GCT | CCA | GTA | CGG | GAA | TAA | 5.0–5.8 | Moderate | |
 |  |  | Asn | Ala | Pro | Val | Arg | Glu | * |  |  |
COLONY PICKING | Â | Variants with High Activity at Low pH | Â | Â | |||||||
MAN | AUTO | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
E7 |  | From G2 well of 768-clone multiplex screen | AAT | GCT | TTA | TAA |  |  |  | 4.0–5.8 | Not Stable |
 |  |  | Asn | Ala | Leu | * |  |  |  |  |  |
F2 | G12 | From B11 well of 768-clone multiplex screen | AAT | GCT | CAA | TGG | ACA | CCA | TAA | 4.0–5.8 | Moderate |
 |  |  | Asn | Ala | Gln | Trp | Thr | Pro | * |  |  |
F9 φ | G4 φ | From G2 well of 768-clone multiplex screen | AAT | GCT | ATG | CCA | ACG | TTA | TAA | 4.0–5.8 | Very Stable |
 |  |  | Asn | Ala | Met | Pro | Thr | Leu | * |  |  |
F12 |  | From G11 well of 768-clone multiplex screen | AAT | GCT | CCA | TAA |  |  |  | 4.0–5.8 | Moderate |
 |  |  | Asn | Ala | Pro | * |  |  |  |  |  |
HTS Mutagenesis | Â | Â | NNR/Y | NNR/Y | NNR/Y | NNR/Y | * | Â | Â | ||
 | C7 | From Plate 2 well of 23,424- clone multiplex screen | AAT | GCT | GGG | TTA | GAA | TAG | TAA | 4.0–5.8 | Very Stable |
 |  |  | Asn | Ala | Gly | Leu | Glu | * | * |  |  |