Ontogenetic variations in the venom proteome of the Amazonian snake Bothrops atrox

Rafael AP Guércio; Anna Shevchenko; Andrej Shevchenko; Jorge L López-Lozano; Jaime Paba; Marcelo V Sousa; Carlos AO Ricart

doi:10.1186/1477-5956-4-11

Proteome Science

Table 1 Protein groups identified in B. atrox venom proteome. Groups from A to Δ comprise spots that displayed similar peptide mass fingerprints shown in Fig 3. pI range, molecular mass (MM) and relative expression were determined by computational analyses of the 2-DE gels. X: unknown amino acid; B: cleavage site; J: juveniles; S: sub-adults, A: adults.

From: Ontogenetic variations in the venom proteome of the Amazonian snake Bothrops atrox

Group	Protein Class	Best Match sptrembl # Organism	Peptide sequences	pI range	MM (kDa)	Relative expression
A	Metalloproteinase	Bothropasin O93523 Bothrops jararaca	BITVKPDVDYTLNSFAEWR BASMSECDPAEHCTGQSSECPADVFHK BMYELANIVNEIFR BKIPCAPEDVK BKTDLLTR BGMVLPGTK BXXFQDVYEAEDSCFK QYNPFR YLEFLLVVDQLLNK BYNPFR	5.0	52	J = S = A
B	L-amino oxidase	L-amino oxidase I Q6TGQ9 Bothrops jararacussu	BXXGQLYEESLQK BLFLTMNK BXXTVTYQAVMK BIKFEPPLPPKK BRFDEIVGGMDK BHDDIFAYEK BXXWYANLGPMRLPEK BFWEDDGIHGGK BETDYEEFLEIAK	5.9 –6.2	55 – 61	J = S = A
C	Metalloproteinase	Bothropasin O93523 Bothrops jararaca	BXXVEVGEECDCGSPR BXXGTECQAA DMADLCTGR BLYCVDSAVNGR BLYCVDSSPANK BGMVLPGTK BKIPCAPEDVK BYNPFR BXXFQDVYEAEDSCFK	7.1–7.5	40 – 51	J>S>A
D	Metalloproteinase	Metalloproteinase Q8QG89 Bothrops insularis	BTLDSFGEWR-BTLDSFEGWR BTLDSFWWR BTLDSFWEGR BTLDSFWGER BYVDLFLVVDHGVLDNK BYVDLFLVVDHGMFMK- BYVDLFLVVDHGXXXK BDLINVQQDSR BENPQCILNKR	5.3 – 6.4	23–24	J<S<A
E	Serine proteinase	Bilineobin Q9PSN3 Agkistrodon bilineatus	BVVGGDECNINEHR BSLPSSPPSVWSASK BSLPSSPPSVXXSASK BDIMLIR BVMGWGSLSSPK BFLCNPR BFLCGGPR BIFLTCTK	6.3 –6.4	28 – 29	J = S = A
F	CRISP	Ablomin Q8JI40 Agkistrodon halys blomhoffi	BSVNPTASNMLK BMEWYPEAAANAER BKPEIQNEIVDLHNSLR BSGPPCGDCPSACDDGLCTNPCTK AGCAAAYCPSSSYK BXXDFDSESPR	7.1 –8.1	28 – 29	J>S = A
G	Phospholipase A₂	Phospholipase A₂ Q7ZTA7 Crotalus viridis	BVAVLCFR FVHDCCYGK BMDLYTLHR-BMDLYTYDK	8.1	16	J = S = A
H	C-type lectin	Botrocetin β chain P22030 Bothrops jararaca Platelet glycoprotein IB-binding protein Q9PSM5 Bothrops jararaca Phospholipase A₂ Q8QG87 Bothrops insularis	BDCPSGWSSYEGSCYK FVVTEYECVASK BXXSFEWSDGTDLSYK BXXTQLAYVVCK -BLAYVVCEAQR -BLAYVVCK BTCLGLEEDSGFR BSLTSPMLK DNQWLSFPCTR BXXNAWLGLWEGK BXXSFEWSDGTDLTYK BXXWADAESLCALQR BTCLGLEEDSGFRK BFCTLQQR BLLQLLQR BEEADFVR BNFVCEFQA BWWIIPCTR BVAAICFR BVAATCFR BYWLYGAK	4.8	17	J = S<A
I	C-type lectin	Botrocetin α chain P22029 Bothrops jararaca	BDCPSGWSSYEGNCYK BLYSGEADFVGDLVTK BWSDGSSVSYENVVER BMNWADAER BKCFALEK BGGHLVSIK BCFALEK BNPFVCK BFFQQK	5.3	15	J = S<A
J	Phospholipase A₂	Myotoxin I P20474 Bothrops asper	BAAAVCFR BSLIEFAK BMILEETK BKSGVIICGEGTPCEK BXXAYPDLFCK BLYSGEADFVVK BVAVLCFR BAAGLCGFR BVTGVPTYK BSGVIICGE	7.8	17	J = S<A
K	Phospholipase A₂	Myotoxin III Q9PVE3 Bothrops asper	BXXVCDENNPCLK BXXVCDENNPPGR-VCDENNPCLK BYFAYPDLFCK VTSYQY BMILQETGKNPVTSYGAYGCNCGVLGR BELCECDKAVAICLR BAVAICLR BYSYSWK	7.8	17	J = S<A
L	Metalloproteinase	Berythractivase Q8UVG0 Bothrops erythromelas	BLTPGSQCADGLCCDQCR BKYVEFVVVLDHGMYK BVPLTGLELWSDR BLYCFLYSSK VVFEPFK-VVQHQVR BVPLTXVLDHRFK KIPCAPEDVK	5.0 –5.9	61 – 66	J>S>A
M	Metalloproteinase	BOJUMET II Q7T1T5 Bothrops jararacusu	BETVLLNR BYLIDNRPPCILNIPLR BFALVGLEMWSNR BFALVGLDMGWSNR BSSDLGMVDLASK VQGPLGNTLTCM PTDTDFDGTLLGLAWR TDTDFDGTLLGLAWR GQSADCPTDDLQR	5.4 –5.7	51–53	J>S>A
N	Serine proteinase	Serine proteinase Q7T229 Bothrops jararacusu	BLVGGDECNINEHR BIMGWGTLSPTK BFLCNPR BVSDYTEWLK	5.0 –5.3	29 – 30	J>S = A
O	Metalloproteinase	Metalloproteinase Q8AWX7 Agkistrodon halys palas	BYIELVIVADHR BSVGIVRDYR BSVANDDEVIRYPK	5.2 –5.4	36 – 37	J>S>A
P	Metalloproteinase	Factor X activator heavy chain Q7T046 Vipera lebetina	BLYETVNALNVLCR BYSVGLVQDYR BYIELVIVADHR BLNLNPDEQR	5.8 –6.2	38 – 40	J>S>A
Q	Serine proteinase	Serine proteinase Q8QG86 Bothrops insularis	BFLAFLYPGR BIYLGIHAR BDIMLIR BLHEPALYTK BLQGLVSDHR BSVANDDP-BSVANDDEE	6.3 –7.4	32 – 34	J = S>A
R	Serine proteinase	Catroxase II Q8QHK2 Crotalus atrox	BLDDVLDQDLG BTLCAGILEGGK BAAYPELPATSR BAAYPERFTSR BLPSNPPW-BLPSNPPWHR BLPSNPPXXXR	6.5	29	J = S>A
S	C-type lectin	Alboaggregin A subunit 2 P81112 Trimeresurus albolabris	WADAER SYENWTEAELK BTCLGLEEDSGFR BTCLALEEDSGFR FCAGYLENK BTPLNLNCR BXXLNLNCR BXXTADAER	4.8	17	J>S = A
T	C-type lectin	Mucrocetin Q6TPG9 Trimeresurus mucrosquamatus	BDCPSGWSSYEGSCYK BFCTQQQTNHLVSFQSR BXXCQFVVTEYPSFQSK BXXSFEWSDGTDLSYK BXXTQLAYVVCK BTTENQWWSR BXXLNLNCR BLFLQQNK BLTSPLLR BMNWEDAEK	4.8	15	J = S>A
U	C-type lectin	Alboagregrina A P81114 Trimeresurus albolabris	BDCPSDWSSYEGHCYR BVFNEPQNWADAEK BQSSEEADFVLK BSSEEADFVLK BXXLDQLLK BLFLQQNK	5.1–5.4	14	J = S>A
V	C-type lectin	Bothrocetin α chain P22029 Bothrops jararaca	BCFVLER BXXSDGSCVCYENLVER BEGFLTWR BSPMSPDTEEGK BXXCYENLVER	5.1–5.4	14	J = S>A
X	VEGF	Vascular Endothelial growth factor Q90X24 Bothrops insularis	CCTDESLECTATGK BLEVMKFTEHTNCECR BETLVSLLEEHPDE PSCVTALR BLFRAVLFNALR	5.6 –6.3	13	J>S>A
W	C-type lectin	Botrocetin α chain P22029 Bothrops jararaca	BDCPSGWSSYEGHCYR BXXWSDGSSVSYENLVER VSFQSDWTDFVVK BLVSFQSDWTDFVVK BLWADAER BNPFVCK BFCSEQAK BNIQSSDLYAWIGLR	5.9 –6.4	15	J = S>A
Y	Phospholipase A₂	Phospholipase A₂ Q8QG87 Bothrops insularis	BYFSYGCYCGLGGLGQPR-GSYGCYCLGGL BXXFVHDCCYGK BXXXFVHDCCYGK BXXKDTYNLQYWLYQK-BXXKDTYNLKYWLYAGK BLTYNLQYWLYQK-BLTYNLKYWLYAAGK BYGEGLYQK-BYGEGLYAGK BVVTTCFR BVAVLCTR BQLCECDFVA BXXCECDFV BXXLWQFGT	6.7	15	J = S>A
Z	C-type lectin e NGF	Platelet glycoprotein IB-binding protein α chain Q9PSM6 Bothrops jararaca Nerve growth factor Q9DEZ9 Crotalus durissus terrificus	BQYFFETK BALTMEGNQASWR BIDTACVCVISR BXXALGQK BFIRPR BNPFVCK BFFQQK BDTPFECPSDWSTHR BXXSDGSCVCYENLVR	7.8	17	J = S>A
Δ	Phospholipase A₂	Myotoxin III Q9PVE3 Bothrops asper	BSYAAYGCNCGVLGR BMLLLETGKLPAK NLWQLGK VAVLCFR BAVAICLR BYSYSWK BYNYLKPFCK BTIVCGENNSCLK	6.4 –8.1	16	J = S>A

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ISSN: 1477-5956

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