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Table 1 Protein groups identified in B. atrox venom proteome. Groups from A to Δ comprise spots that displayed similar peptide mass fingerprints shown in Fig 3. pI range, molecular mass (MM) and relative expression were determined by computational analyses of the 2-DE gels. X: unknown amino acid; B: cleavage site; J: juveniles; S: sub-adults, A: adults.

From: Ontogenetic variations in the venom proteome of the Amazonian snake Bothrops atrox

Group Protein Class Best Match sptrembl # Organism Peptide sequences pI range MM (kDa) Relative expression
A Metalloproteinase Bothropasin
O93523
Bothrops jararaca
BITVKPDVDYTLNSFAEWR
BASMSECDPAEHCTGQSSECPADVFHK
BMYELANIVNEIFR
BKIPCAPEDVK
BKTDLLTR
BGMVLPGTK
BXXFQDVYEAEDSCFK
QYNPFR
YLEFLLVVDQLLNK
BYNPFR
5.0 52 J = S = A
B L-amino
oxidase
L-amino oxidase I
Q6TGQ9
Bothrops jararacussu
BXXGQLYEESLQK
BLFLTMNK
BXXTVTYQAVMK
BIKFEPPLPPKK
BRFDEIVGGMDK
BHDDIFAYEK
BXXWYANLGPMRLPEK
BFWEDDGIHGGK
BETDYEEFLEIAK
5.9 –6.2 55 – 61 J = S = A
C Metalloproteinase Bothropasin
O93523
Bothrops jararaca
BXXVEVGEECDCGSPR
BXXGTECQAA
DMADLCTGR
BLYCVDSAVNGR
BLYCVDSSPANK
BGMVLPGTK
BKIPCAPEDVK
BYNPFR
BXXFQDVYEAEDSCFK
7.1–7.5 40 – 51 J>S>A
D Metalloproteinase Metalloproteinase
Q8QG89
Bothrops insularis
BTLDSFGEWR-BTLDSFEGWR
BTLDSFWWR
BTLDSFWEGR
BTLDSFWGER
BYVDLFLVVDHGVLDNK
BYVDLFLVVDHGMFMK-
BYVDLFLVVDHGXXXK
BDLINVQQDSR
BENPQCILNKR
5.3 – 6.4 23–24 J<S<A
E Serine proteinase Bilineobin
Q9PSN3
Agkistrodon bilineatus
BVVGGDECNINEHR
BSLPSSPPSVWSASK
BSLPSSPPSVXXSASK
BDIMLIR
BVMGWGSLSSPK
BFLCNPR
BFLCGGPR
BIFLTCTK
6.3 –6.4 28 – 29 J = S = A
F CRISP Ablomin
Q8JI40
Agkistrodon halys blomhoffi
BSVNPTASNMLK
BMEWYPEAAANAER
BKPEIQNEIVDLHNSLR
BSGPPCGDCPSACDDGLCTNPCTK
AGCAAAYCPSSSYK
BXXDFDSESPR
7.1 –8.1 28 – 29 J>S = A
G Phospholipase A2 Phospholipase A2
Q7ZTA7
Crotalus viridis
BVAVLCFR
FVHDCCYGK
BMDLYTLHR-BMDLYTYDK
8.1 16 J = S = A
H C-type lectin Botrocetin β chain
P22030
Bothrops jararaca
Platelet glycoprotein
IB-binding protein
Q9PSM5
Bothrops jararaca
Phospholipase A2
Q8QG87
Bothrops insularis
BDCPSGWSSYEGSCYK
FVVTEYECVASK
BXXSFEWSDGTDLSYK
BXXTQLAYVVCK
-BLAYVVCEAQR
-BLAYVVCK
BTCLGLEEDSGFR
BSLTSPMLK
DNQWLSFPCTR
BXXNAWLGLWEGK
BXXSFEWSDGTDLTYK
BXXWADAESLCALQR
BTCLGLEEDSGFRK
BFCTLQQR
BLLQLLQR
BEEADFVR
BNFVCEFQA
BWWIIPCTR
BVAAICFR
BVAATCFR
BYWLYGAK
4.8 17 J = S<A
I C-type lectin Botrocetin α chain
P22029
Bothrops jararaca
BDCPSGWSSYEGNCYK
BLYSGEADFVGDLVTK
BWSDGSSVSYENVVER
BMNWADAER
BKCFALEK
BGGHLVSIK
BCFALEK
BNPFVCK
BFFQQK
5.3 15 J = S<A
J Phospholipase A2 Myotoxin I
P20474
Bothrops asper
BAAAVCFR
BSLIEFAK
BMILEETK
BKSGVIICGEGTPCEK
BXXAYPDLFCK
BLYSGEADFVVK
BVAVLCFR
BAAGLCGFR
BVTGVPTYK
BSGVIICGE
7.8 17 J = S<A
K Phospholipase A2 Myotoxin III
Q9PVE3
Bothrops asper
BXXVCDENNPCLK
BXXVCDENNPPGR-VCDENNPCLK
BYFAYPDLFCK
VTSYQY
BMILQETGKNPVTSYGAYGCNCGVLGR
BELCECDKAVAICLR
BAVAICLR
BYSYSWK
7.8 17 J = S<A
L Metalloproteinase Berythractivase
Q8UVG0
Bothrops erythromelas
BLTPGSQCADGLCCDQCR
BKYVEFVVVLDHGMYK
BVPLTGLELWSDR
BLYCFLYSSK
VVFEPFK-VVQHQVR
BVPLTXVLDHRFK
KIPCAPEDVK
5.0 –5.9 61 – 66 J>S>A
M Metalloproteinase BOJUMET II
Q7T1T5
Bothrops jararacusu
BETVLLNR
BYLIDNRPPCILNIPLR
BFALVGLEMWSNR
BFALVGLDMGWSNR
BSSDLGMVDLASK
VQGPLGNTLTCM
PTDTDFDGTLLGLAWR
TDTDFDGTLLGLAWR
GQSADCPTDDLQR
5.4 –5.7 51–53 J>S>A
N Serine proteinase Serine proteinase
Q7T229
Bothrops jararacusu
BLVGGDECNINEHR
BIMGWGTLSPTK
BFLCNPR
BVSDYTEWLK
5.0 –5.3 29 – 30 J>S = A
O Metalloproteinase Metalloproteinase
Q8AWX7
Agkistrodon halys palas
BYIELVIVADHR
BSVGIVRDYR
BSVANDDEVIRYPK
5.2 –5.4 36 – 37 J>S>A
P Metalloproteinase Factor X activator
heavy chain
Q7T046
Vipera lebetina
BLYETVNALNVLCR
BYSVGLVQDYR
BYIELVIVADHR
BLNLNPDEQR
5.8 –6.2 38 – 40 J>S>A
Q Serine proteinase Serine proteinase
Q8QG86
Bothrops insularis
BFLAFLYPGR
BIYLGIHAR
BDIMLIR
BLHEPALYTK
BLQGLVSDHR
BSVANDDP-BSVANDDEE
6.3 –7.4 32 – 34 J = S>A
R Serine proteinase Catroxase II
Q8QHK2
Crotalus atrox
BLDDVLDQDLG
BTLCAGILEGGK
BAAYPELPATSR
BAAYPERFTSR
BLPSNPPW-BLPSNPPWHR
BLPSNPPXXXR
6.5 29 J = S>A
S C-type lectin Alboaggregin A
subunit 2
P81112
Trimeresurus albolabris
WADAER
SYENWTEAELK
BTCLGLEEDSGFR
BTCLALEEDSGFR
FCAGYLENK
BTPLNLNCR
BXXLNLNCR
BXXTADAER
4.8 17 J>S = A
T C-type lectin Mucrocetin
Q6TPG9
Trimeresurus mucrosquamatus
BDCPSGWSSYEGSCYK
BFCTQQQTNHLVSFQSR
BXXCQFVVTEYPSFQSK
BXXSFEWSDGTDLSYK
BXXTQLAYVVCK
BTTENQWWSR
BXXLNLNCR
BLFLQQNK
BLTSPLLR
BMNWEDAEK
4.8 15 J = S>A
U C-type lectin Alboagregrina A
P81114
Trimeresurus albolabris
BDCPSDWSSYEGHCYR
BVFNEPQNWADAEK
BQSSEEADFVLK
BSSEEADFVLK
BXXLDQLLK
BLFLQQNK
5.1–5.4 14 J = S>A
V C-type lectin Bothrocetin α chain
P22029
Bothrops jararaca
BCFVLER
BXXSDGSCVCYENLVER
BEGFLTWR
BSPMSPDTEEGK
BXXCYENLVER
5.1–5.4 14 J = S>A
X VEGF Vascular Endothelial
growth factor
Q90X24
Bothrops insularis
CCTDESLECTATGK
BLEVMKFTEHTNCECR
BETLVSLLEEHPDE
PSCVTALR
BLFRAVLFNALR
5.6 –6.3 13 J>S>A
W C-type lectin Botrocetin α chain
P22029
Bothrops jararaca
BDCPSGWSSYEGHCYR
BXXWSDGSSVSYENLVER
VSFQSDWTDFVVK
BLVSFQSDWTDFVVK
BLWADAER
BNPFVCK
BFCSEQAK
BNIQSSDLYAWIGLR
5.9 –6.4 15 J = S>A
Y Phospholipase A2 Phospholipase A2
Q8QG87
Bothrops insularis
BYFSYGCYCGLGGLGQPR-GSYGCYCLGGL
BXXFVHDCCYGK
BXXXFVHDCCYGK
BXXKDTYNLQYWLYQK-BXXKDTYNLKYWLYAGK
BLTYNLQYWLYQK-BLTYNLKYWLYAAGK
BYGEGLYQK-BYGEGLYAGK
BVVTTCFR
BVAVLCTR
BQLCECDFVA
BXXCECDFV
BXXLWQFGT
6.7 15 J = S>A
Z C-type lectin
e NGF
Platelet glycoprotein
IB-binding protein
α chain
Q9PSM6
Bothrops jararaca
Nerve growth factor
Q9DEZ9
Crotalus durissus terrificus
BQYFFETK
BALTMEGNQASWR
BIDTACVCVISR
BXXALGQK
BFIRPR
BNPFVCK
BFFQQK
BDTPFECPSDWSTHR
BXXSDGSCVCYENLVR
7.8 17 J = S>A
Δ Phospholipase A2 Myotoxin III
Q9PVE3
Bothrops asper
BSYAAYGCNCGVLGR
BMLLLETGKLPAK
NLWQLGK
VAVLCFR
BAVAICLR
BYSYSWK
BYNYLKPFCK
BTIVCGENNSCLK
6.4 –8.1 16 J = S>A