A : increasing attached peptide at four fixed levels of added antibody | ||||
---|---|---|---|---|
 | 1 stAB Concentration 0.0005 serum/PBS | 1 stAB Concentration 0.001 serum/PBS | 1 stAB Concentration 0.005 serum/PBS | 1 stAB Concentration 0.01 serum/PBS |
Kd (μg/ml) | 0.49 ± 0.25 | 0.89 ± 0.56 | 0.49 ± 0.097 | 0.24 ± 0.06 |
Amax (OD units) | 0.076 ± 0.01 | 0.12 ± 0.01 | 0.22 ± 0.012 | 0.35 ± 0.024 |
D (OD units) | 0.29 ± 0.008 | 0.3 ± 0.015 | 0.29 ± 0.009 | 0.3 ± 0.02 |
B: increasing added antibody at three fixed levels of attached peptide | ||||
 | Attached peptide 0.25 μg/ml | Attached peptide 0.5 μg/ml | Attached peptide 1 μg/ml |  |
Kd (serum dilutions) | 0.003 ± 0.0007 | 0.003 ± 0.001 | 0.001 ± 0.0002 |  |
Amax (OD units) | 0.35 ± 0.023 | 0.56 ± 0.06 | 0.49 ± 0.02 |  |
D (OD units) | 0.38 ± 0.01 | 0.38 ± 0.02 | 0.38 ± 0.01 |  |
C: Competition assay – Kd determined when increasing added free peptide at two fixed levels of attached peptide | ||||
 | Attached peptide 0.25 μg/ml | Attached peptide 0.5 μg/ml |  |  |
Kd (μg/ml) | 0.14 ± 0.03 | 0.97 ± 0.15 |  |  |
Amax (OD units) | 0.29 ± 0.019 | 0.37 ± 0.016 |  |  |
D (OD units) | 0.39 ± 0.01 | 0.35 ± 0.01 |  |  |