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Table 2 Kinetic parameters obtained from the optimization of the Peptidomatrix conditions for peptide MXR P2 and the serum from rabbit # 64850 (see Figure 2) (± SD, n = 3).

From: Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

A : increasing attached peptide at four fixed levels of added antibody
  1 stAB Concentration 0.0005 serum/PBS 1 stAB Concentration 0.001 serum/PBS 1 stAB Concentration 0.005 serum/PBS 1 stAB Concentration 0.01 serum/PBS
Kd (μg/ml) 0.49 ± 0.25 0.89 ± 0.56 0.49 ± 0.097 0.24 ± 0.06
Amax (OD units) 0.076 ± 0.01 0.12 ± 0.01 0.22 ± 0.012 0.35 ± 0.024
D (OD units) 0.29 ± 0.008 0.3 ± 0.015 0.29 ± 0.009 0.3 ± 0.02
B: increasing added antibody at three fixed levels of attached peptide
  Attached peptide 0.25 μg/ml Attached peptide 0.5 μg/ml Attached peptide 1 μg/ml  
Kd (serum dilutions) 0.003 ± 0.0007 0.003 ± 0.001 0.001 ± 0.0002  
Amax (OD units) 0.35 ± 0.023 0.56 ± 0.06 0.49 ± 0.02  
D (OD units) 0.38 ± 0.01 0.38 ± 0.02 0.38 ± 0.01  
C: Competition assay – Kd determined when increasing added free peptide at two fixed levels of attached peptide
  Attached peptide 0.25 μg/ml Attached peptide 0.5 μg/ml   
Kd (μg/ml) 0.14 ± 0.03 0.97 ± 0.15   
Amax (OD units) 0.29 ± 0.019 0.37 ± 0.016   
D (OD units) 0.39 ± 0.01 0.35 ± 0.01