A: increasing attached peptide at two fixed levels of added antibody | |||
---|---|---|---|
 | 1 stAB Concentration 0.00033 serum/PBS | 1 stAB Concentration 0.00066 serum/PBS |  |
Kd (μg/ml) | 0.19 ± 0.01 | 0.1 ± 0.02 |  |
Amax (OD units) | 1.36 ± 0.02 | 1.56 ± 0.08 |  |
D (OD units) | 0.25 ± 0.02 | 0.38 ± 0.07 |  |
B: Competition assay – Kd determined when increasing added free peptide at three fixed levels of added antibody | |||
 | 1 stAB concentration 0.000625 serum/PBS | 1 stAB concentration 0.000125serum/PBS | 1 stAB concentration 0.0025 serum/PBS |
Kd (μg/ml) | 0.55 ± 0.1 | 0.83 ± 0.12 | 2.55 ± 1.21 |
Amax (OD units) | 1.16 ± 0.05 | 1.35 ± 0.05 | 1.33 ± 0.17 |
D (OD units) | 0.59 ± 0.04 | 0.86 ± 0.04 | 1.17 ± 0.18 |
C: increasing attached peptide at two fixed levels of added antibody | |||
 | 1 stAB concentration 0.00033 serum/PBS | 1 stAB concentration 0.00067 serum/PBS |  |
Kd (serum dilutions) | 0.33 ± 0.08 | 0.29 ± 0.04 |  |
Amax (OD units) | 1.46 ± 0.09 | 1.74 ± 0.06 |  |
D (OD units) | 0.19 ± 0.079 | 0.24 ± 0.05 |  |
D: Competition assay- Kd determined when increasing added free peptide at three fixed levels of added antibody | |||
 | 1 stAB concentration 0.000625 serum/PBS | 1 stAB concentration 0.00125 serum/PBS | 1 stAB concentration 0.0025 serum/PBS |
Kd (μg/ml) | 0.68 ± 0.12 | 0.99 ± 0.19 | 2.04 ± 0.3 |
Amax (OD units) | 1.58 ± 0.06 | 1.87 ± 0.09 | 2.05 ± 0.08 |
D (OD units) | 0.25 ± 0.05 | 0.25 ± 0.08 | 0.28 ± 0.08 |