|
1 stAB Concentration 0.00033 serum/PBS
|
1 stAB Concentration 0.00066 serum/PBS
| |
Kd (μg/ml)
|
0.19 ± 0.01
|
0.1 ± 0.02
| |
Amax (OD units)
|
1.36 ± 0.02
|
1.56 ± 0.08
| |
D (OD units)
|
0.25 ± 0.02
|
0.38 ± 0.07
| |
B: Competition assay – Kd determined when increasing added free peptide at three fixed levels of added antibody
|
|
1 stAB concentration 0.000625 serum/PBS
|
1 stAB concentration 0.000125serum/PBS
|
1 stAB concentration 0.0025 serum/PBS
|
Kd (μg/ml)
|
0.55 ± 0.1
|
0.83 ± 0.12
|
2.55 ± 1.21
|
Amax (OD units)
|
1.16 ± 0.05
|
1.35 ± 0.05
|
1.33 ± 0.17
|
D (OD units)
|
0.59 ± 0.04
|
0.86 ± 0.04
|
1.17 ± 0.18
|
C: increasing attached peptide at two fixed levels of added antibody
|
|
1 stAB concentration 0.00033 serum/PBS
|
1 stAB concentration 0.00067 serum/PBS
| |
Kd (serum dilutions)
|
0.33 ± 0.08
|
0.29 ± 0.04
| |
Amax (OD units)
|
1.46 ± 0.09
|
1.74 ± 0.06
| |
D (OD units)
|
0.19 ± 0.079
|
0.24 ± 0.05
| |
D: Competition assay- Kd determined when increasing added free peptide at three fixed levels of added antibody
|
|
1 stAB concentration 0.000625 serum/PBS
|
1 stAB concentration 0.00125 serum/PBS
|
1 stAB concentration 0.0025 serum/PBS
|
Kd (μg/ml)
|
0.68 ± 0.12
|
0.99 ± 0.19
|
2.04 ± 0.3
|
Amax (OD units)
|
1.58 ± 0.06
|
1.87 ± 0.09
|
2.05 ± 0.08
|
D (OD units)
|
0.25 ± 0.05
|
0.25 ± 0.08
|
0.28 ± 0.08
|