Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

Table 4 summary of the results with the different peptides.

	A- peptide titration		B- serum titration		C-competition (peptide titration)		D-competition (Antibody titration)
	Kp	Amax	Ka	Amax	Kd	Amax	Kd	Amax
1	↓	↑	↓	↑	↑	↑
2	↓	↑	↓	↑	↑	↑
3	↓	↑	↔	↑	↑	↑
4	↓	↑	↓	↑	↑	↑
5	↓	↑	↓	↑
6	↓	↑			↑	↑	↑	↑
7	↓	↑					↑	↑
8	↓	↑					↑	↑
9	↓	↑					↑	↑
10					↑	↑	↑	↑
11	↓	↑			↑	↑	↑	↑

A-the effect of increasing the amount of antibody.
B- the effect of increasing the amount of attached peptide.
C- the effect of increasing the amount of attached peptide on the parameters of the competition.
D- the effect of increasing the amount of antibody on the parameters of the competition.
(1)MXR P5/#853, (2) MXR P5/#851, (3) MXR P2/#850, (4) MXR P2/#795, (5) MXR P3/#795, (6) MXR P1/#841, (7) ATP P1/#840, (8) ATP P3/#855, (9) ATP P4/# 485, (10) MRP P1/#151 #152,(11) MRP P2/#151 & #152