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Table 5 Kinetic parameters obtained by simulating the conditions for the Peptidomatrix using equations A2 and A5 (single binding site). Data in Fig 5.

From: Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

A : increasing attached peptide at four fixed levels of added antibody
  Antibody 0.1 Antibody 1 Antibody 10 Antibody 100
Kd 30.00 30.00 30.00 30.00
Amax 0.5 4.55 25.00 45.45
B: increasing added antibody at four fixed levels of attached peptide
  Attached peptide 0.1 Attached peptide 1 Attached peptide 10 Attached peptide 100
Kd 100.00 100.00 100.00 100.00
Amax 1.61 12.50 38.46 48.54
C: Competition assay – Kd determined when increasing added free peptide at four fixed levels of attached peptide
  Attached peptide 0.1 Attached peptide 1 Attached peptide 10 Attached peptide 100
Kd 1.10 1.10 1.10 1.10
Amax 0.15 1.14 3.5 4.41
D: Competition assay – Kd determined when increasing free peptide at four fixed levels of added antibody
  Antibody 0.1 Antibody 1 Antibody 10 Antibody 100
Kd 1.01 1.10 2.00 11.00
Amax 0.12 1.14 6.25 11.36