Table 5 Kinetic parameters obtained by simulating the conditions for the Peptidomatrix using equations A2 and A5 (single binding site). Data in Fig 5.
A : increasing attached peptide at four fixed levels of added antibody | ||||
|---|---|---|---|---|
| Â | Antibody 0.1 | Antibody 1 | Antibody 10 | Antibody 100 |
Kd | 30.00 | 30.00 | 30.00 | 30.00 |
Amax | 0.5 | 4.55 | 25.00 | 45.45 |
B: increasing added antibody at four fixed levels of attached peptide | ||||
| Â | Attached peptide 0.1 | Attached peptide 1 | Attached peptide 10 | Attached peptide 100 |
Kd | 100.00 | 100.00 | 100.00 | 100.00 |
Amax | 1.61 | 12.50 | 38.46 | 48.54 |
C: Competition assay – Kd determined when increasing added free peptide at four fixed levels of attached peptide | ||||
| Â | Attached peptide 0.1 | Attached peptide 1 | Attached peptide 10 | Attached peptide 100 |
Kd | 1.10 | 1.10 | 1.10 | 1.10 |
Amax | 0.15 | 1.14 | 3.5 | 4.41 |
D: Competition assay – Kd determined when increasing free peptide at four fixed levels of added antibody | ||||
| Â | Antibody 0.1 | Antibody 1 | Antibody 10 | Antibody 100 |
Kd | 1.01 | 1.10 | 2.00 | 11.00 |
Amax | 0.12 | 1.14 | 6.25 | 11.36 |