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Table 6 Kinetic parameters obtained by simulating the conditions for the Peptidomatrix, using equations A2 and A7 (two binding sites). Data in Fig 6.

From: Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

A: increasing attached peptide at four fixed levels of added antibody
  Antibody 0.1 Antibody 1 Antibody 10 Antibody 100
Kd 25.53 25.22 22.69 14.70
Amax 0.52 4.80 27.49 61.77
B: increasing added antibody at four fixed levels of attached peptide
  Attached peptide 0.1 Attached peptide 1 Attached peptide 10 Attached peptide 100
Kd 70.55 65.62 43.28 25.68
Amax 1.58 12.28 41.07 69.42
C: Competition assay – Kd determined when increasing added free peptide at four fixed levels of attached peptide
  Attached peptide 0.1 Attached peptide 1 Attached peptide 10 Attached peptide 100
Kd 1.3869 1.4333 1.6399 1.8080
Amax 0.2291 1.9375 9.7190 23.5973
D: Competition assay – Kd determined when increasing free peptide at four fixed levels of added antibody
  Antibody 0.1 Antibody 1 Antibody 10 Antibody 100
Kd 1.001 1.01 1.64 4.12
Amax 0.16 1.52 9.72 28.98