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Figure 1 | Proteome Science

Figure 1

From: Proteome-level display by 2-dimensional chromatography of extracellular matrix-dependent modulation of the phenotype of bladder cancer cells

Figure 1

"Gel-view" proteomic display of J82 cells cultured on Matrigel (A), SISgel (B) and plastic (C). Due to the current software configuration, the elution is from right to left, with lanes 17–25 showing proteins eluted during the base wash, lanes 2–16 showing proteins eluting during the pH 8.3-4.0 gradient, and lane 1 showing proteins eluting in the 1.0 M NaCl wash. The Y axis shows the retention times of the second dimension between 10 and 24 minutes. The software presents the elution pattern as a simulated gel-view in which the color intensity is proportional to the absorbance. On the left of each display is shown a chromatographic view of one lane from which fractions were obtained for MS analysis presented as A216 vs retention time. The black arrows identify fractions taken for MS analysis and reported in Table 1.

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