Table 1 Identification of several proteins unique to one or more samples. Only those for which a MOWSE score of >65 (p = 0.05)for PMF or >34 (p = 0.05) for MS/MS and for which matching sequences spanned the entire sequence are reported. The experimental pI is the measured pH value for the first dimension fraction. The # Seq. Matched represents the number of fragments matching peptide sequences.
Exp. pI | RT 2nd Dim. (min) | Gene Symbol | NCBI Accession Number | Sequence MW/pI | MOWSE Score (PMF) | Coverage % (# Seq. Matched) | MOWSE Score (MS/MS) |
|---|---|---|---|---|---|---|---|
Cells grown on Matrigel | |||||||
<4.0 | 17.560 | ER60 HSPA5 VIME | 2245365 16507237 37852 | 57245/5.88 72431/5.07 53724/5.06 | 81 68 86 | 29 (13) 23 (11) 28 (13) | 94 |
<4.0 | 18.027 | HSPA5 | 16507237 | 72431/5.07 | 120 | 29 (17) | |
<4.0 | 18.777 | HSPA8 HSPA5 ENOA | 24234686 16507237 1167843 | 53626/5.62 72431/5.07 47566/7.01 | 103 136 96 | 25(7) 22(11) 17(8) | 41 36 |
<4.0 | 20.687 | HSP60 | 49522865 | 61229/5.71 | 99 | 20(8) | 93 |
4.63–4.94 | 17.167 | ATM | 1497931 | 356785/6.37 | 74 | 9 (19) | |
7.02–7.32 | 16.740 | H2B.1 | 1568551 | 13928/10.3 | 106 | 57(9) | 48 |
7.02–7.32 | 16.998 | RPL9 RPS18 | 13278765 75517910 | 21992/9.96 17708/11.0 | 101 73 | 53 (8) 38 (9) | 78 44 |
>8.3 | 16.597 | APC | 68533057 | 314407/8.03 | 70 | 9 (18) | |
>8.3 | 16.687 | ALDOA | 4557305 | 39964/8.33 | 70 | 26 (7) | 46 |
>8.3 | 17.094 | GAPDH | 31645 | 36244/8.26 | 191 | 66 (16) | 64 |
>8.3 | 16.634 | RUVBL/CHTF18 | 14336725 | 130269/9.51 | 72 | 16 (13) | |
Cells grown on SISgel | |||||||
>8.3 | 16.774 | RSNL2 | 48257203 | 60839/9.07 | 70 | 23 (11) | |
5.54–5.84 | 17.667 | AKR1B1 ATPB | 13529257 28931 | 36298/6.82 34026/4.90 | 155 96 | 49 (14) 25 (6) | 74 59 |
5.54–5.84 | 17.695 | AKR1B1 ATPB | 13529257 28931 | 36298/6.82 34026/4.90 | 166 90 | 48 (17) 28 (7) | 68 79 |
5.94–6.13 | 17.667 | AKR1B1 ATPB | 13529257 28931 | 36298/6.82 34026/4.90 | 98 48 | 55 (13) 22 (3) | 51 |
Cells grown on plastic | |||||||
>8.3 | 17.407 | GAPDH | 31645 | 36244/8.26 | 192 | 56 (16) | 68 |