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Figure 2 | Proteome Science

Figure 2

From: An ELISA-based procedure for assaying proteins in digests of human leukocytes and cell lines, using specifically selected peptides and appropriate antibodies

Figure 2

The procedure of the 'Peptidomatrix' assay. The ELISA Peptidomatrix is a competition assay: Peptides are bound to the plastic wells and they are subsequently exposed to antibodies generated against them. A labeled secondary antibody is used to detect the bound primary antibodies. In the assay itself the antibodies are mixed with soluble synthetic peptide in known amounts, to generate a calibration curve. The peptide in solution competes with the bound peptide and will affect the signal. As shown schematically in the figure: Panel A, A': No free peptide is added, thus the signal will be the highest. Panel B, B': A small amount of free peptide is added and the signal will be intermediate. Panel C, C': A large amount of competing peptide is added, thus no antibody binds to the well and the signal is minimal.

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ISSN: 1477-5956