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Figure 8 | Proteome Science

Figure 8

From: Analytical model of peptide mass cluster centres with applications

Figure 8

Principle and results of linear regression on peptide rule LR/PR calibration. Panel A: Scatter-plot of Δ PR (m i , m j ) (Equation 31) in dependence of Δ d = |m i - m j |. The slope, obtained by robust regression, is shown by the red line. Panel B: Histogram (black with diagonals) of d PR (m i , 0). The continuous vertical red line denotes the average ( d ¯ MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaacuWGKbazgaqeaaaa@2E15@ PR (m i , 0)) and the dotted vertical lines denote ( d ¯ MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaacuWGKbazgaqeaaaa@2E15@ PR (m i , 0) ± S N . The histogram in gray is showing the distribution of (d PR (m i , 0) previous to removing the slope error (see text). Panel C & D: Strip-charts of the data-set for a mass range of 2210 – 2212Da and 842 – 843Da, including the tryptic autolysis peaks 842.508Da and 2211.100Da. Gray triangles – raw data; blue "+" – Wool Smilansky algorithm (cf. Appendix); red "o" – LR/RP algorithm for tryptic peaks .

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