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Table 1 Dextransucrase (DsrS) production during a high cell density fed-batch cultivation.

From: Comparative proteomic analysis of high cell density cultivations with two recombinant Bacillus megaterium strains for the production of a heterologous dextransucrase

Time after induction (h) Activity of DsrS (U/L)
  cell-associated Secreted
  soluble fraction insoluble fraction  
0.5 157 30690 n.d.
1.5 415 71700 n.d.
3.0 365 47422 n.d.
  1. Dextransucrase (DsrS) production during a high cell density fed-batch cultivation with B. megaterium strain WH320 carrying the plasmid pMM1520dsrS after induction with xylose, determined as volumetric activities of DsrS according to an activity staining method previously described [6-7].
  2. n.d.: not detectable