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Table 1 Dextransucrase (DsrS) production during a high cell density fed-batch cultivation.

From: Comparative proteomic analysis of high cell density cultivations with two recombinant Bacillus megaterium strains for the production of a heterologous dextransucrase

Time after induction (h)

Activity of DsrS (U/L)

 

cell-associated

Secreted

 

soluble fraction

insoluble fraction

 

0.5

157

30690

n.d.

1.5

415

71700

n.d.

3.0

365

47422

n.d.

  1. Dextransucrase (DsrS) production during a high cell density fed-batch cultivation with B. megaterium strain WH320 carrying the plasmid pMM1520dsrS after induction with xylose, determined as volumetric activities of DsrS according to an activity staining method previously described [6-7].
  2. n.d.: not detectable
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Proteome Science

ISSN: 1477-5956