Signal stability of Cy3 and Cy5 on antibody microarrays

Table 1 Summary of ratio comparisons of Cy3 spots.

Slide Number	$\bar{I_{10} / I_{0}}$	$\bar{I_{20} / I_{0}}$	$\bar{I_{30} / I_{0}}$
1	1.037 ± 0.004	1.086 ± 0.005	1.157 ± 0.006
2	1.008 ± 0.017	1.047 ± 0.004	1.087 ± 0.004
3	0.986 ± 0.003	1.025 ± 0.003	1.006 ± 0.002
4	1.021 ± 0.002	1.093 ± 0.002	0.988 ± 0.002
5	0.973 ± 0.007	1.006 ± 0.008	1.071 ± 0.009
6	1.044 ± 0.006	1.121 ± 0.007	1.104 ± 0.007
7	0.991 ± 0.008	0.990 ± 0.003	0.990 ± 0.003
8	0.982 ± 0.003	0.929 ± 0.003	0.948 ± 0.003
9	1.039 ± 0.004	1.051 ± 0.004	1.060 ± 0.004
10	1.055 ± 0.004	1.064 ± 0.006	1.001 ± 0.004
11	1.084 ± 0.009	1.142 ± 0.004	1.075 ± 0.004
12	1.066 ± 0.003	1.117 ± 0.012	1.026 ± 0.003
13	1.135 ± 0.007	1.138 ± 0.006	1.083 ± 0.021
14	0.985 ± 0.004	1.101 ± 0.003	1.114 ± 0.014
Average	1.029 ± 0.013	1.065 ± 0.018	1.051 ± 0.017

Each of the 1014 microarray spot was examined at day 0, 10, 20, and 30, respectively, and its intensities (I₀, I₁₀, I₂₀, and I₃₀) were measured. Then, the ratios of I₁₀/I₀, I₂₀/I₀, and I₃₀/I₀ for each microarray spot were generated. The average ratios of all 1014 spots on each individual slide at day 10 (I₁₀/I₀), day 20 (I₂₀/I₀), and day 30 (I₃₀/I₀) as well as the averaged value of all slides at day 10, 20 and 30 are shown (mean ± SEM).

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