Effect of long-term exposure of SH-SY5Y cells to morphine: a whole cell proteomic analysis

Table 1 Proteins whose abundance in SH-SY5Y cells is regulated after 6 h of morphine treatment

Spot #	Protein	Accession # (a)	MW (Da)	Variation (% of control) (b)	MALDI peptides	% coverage	MS/MS peptides (c)
2	Elongation factor G 1	Q96RP9	83506	42 ± 11	13	16
3	Ezrin	P15311	69268	165 ± 26	10	15
5	Lamin A/C	P02545	74139	203 ± 21	18	32
9	Bifunctional purine biosynthesis protein	P31939	65088	122 ± 16	14	30
11	no identification			52 ± 10
14	Vacuolar ATP synthase subunit B, brain isoform	P21281	56501	151 ± 17	18	54
15	Rho-GTPase-activating protein 1	Q07960	50461	166 ± 44			4
17	Septin-11	Q9NVA2	49267	141 ± 19	13	37
18	ATP synthase beta chain	P06576	56560	35 ± 9	10	48
24	Rab GDP dissociation inhibitor beta Mitochondrial-processing peptidase beta subunit	P50395 O75439	50663 55072	136 ± 16	14 12	36 25
27	Synaptic vesicle membrane protein VAT-1 homolog	Q99536	41920	141 ± 17	7	31
28	Adenylosuccinate synthetase 2	P30520	50097	147 ± 14			5
36	40 kDa peptidyl-prolyl cis-trans isomerase	Q08752	40632	174 ± 42	12	26
44	Malate dehydrogenase, cytoplasmic	P40925	36295	50 ± 9	11	32
49	Guanine nucleotide binding protein beta subunit 2-like 1	P63244	35077	75 ± 15	15	69
52	Platelet-activating factor acetylhydrolase IB gamma	Q15102	25734	70 ± 14			5
53	Triosephosphate isomerase	P60174	26538	186 ± 60	13	65
54	Ras-related protein Rab-7	P51149	23490	56 ± 13	13	65

^aSwiss-Prot primary accession number
^bMean ± S.D., control refers to untreated cells.
^cOnly peptides with a statistically significant Mascot score (p < 0.05) were considered

ISSN: 1477-5956