Effect of long-term exposure of SH-SY5Y cells to morphine: a whole cell proteomic analysis

Table 2 Proteins whose abundance in SH-SY5Y cells is regulated after 24 h of morphine treatment

Spot #	Protein	Accession # (a)	MW (Da)	Variation (% of control) (b)	MALDI peptides	% coverage	MS/MS peptides (c)
1	ATP-dependent RNA helicase DDX1	Q92499	82432	54 ± 10	27	32
7	Glycerol-3-phosphate dehydrogenase, mitochondrial	P43304	80815	75 ± 7	15	22
9	Bifunctional purine biosynthesis protein	P31939	65088	118 ± 11	14	30
14	Vacuolar ATP synthase subunit B, brain isoform	P21281	56501	131 ± 13	18	54
16	RuvB-like 1	Q9Y265	50228	144 ± 32	16	66
19	4-trimethylaminobutyraldehyde dehydrogenase WD-repeat protein 12	P49189 Q9GZL7	53802 48190	145 ± 24	15 12	32 25
20	Alpha-enolase	P06733	47038	45 ± 15	18	60
21	Alpha-enolase	P06733	47038	184 ± 53			6
22	Histone acetyltransferase type B subunit 2	Q16576	47820	145 ± 65	10	30
30	Heterogeneous nuclear riboprotein F Eucaryotic translation initiation factor 3 subunit 5	O00303	45853 37654	181 ± 60	12 7	42 32
32	Beta-succinyl CoA synthetase	Q7Z503	43611	74 ± 10	15	35
33	Galactokinase	P51570	42272	58 ± 9	15	43
35	Acyl-CoA hydrolase	O00154	41796	166 ± 33	11	44
37	Arsenical pump-driving ATPase	O43681	38793	167 ± 29	8	26
38	Serine-threonine kinase receptor associated protein	Q9Y3F4	38756	164 ± 31	14	48
41	Protein phosphatase 2A, regulatory subunit B' Calponin-3	Q15257 Q15417	40682 36561	65 ± 8	10 7	30 30
42	Biliverdin reductase A	P53004	33428	84 ± 6	12	44
44	Malate dehydrogenase, cytoplasmic	P40925	36295	49 ± 25	11	32
46	Annexin A5	P08758	35806	152 ± 41	18	70
51	Endoplasmic reticulum protein ERp29	P30040	28993	76 ± 14	8	46
54	Ras-related protein Rab-7	P51149	23490	58 ± 12	13	65

^aSwiss-Prot primary accession number
^bMean ± S.D., control refers to untreated cells.
^cOnly peptides with a statistically significant Mascot score (p < 0.05) were considered

ISSN: 1477-5956