Effect of long-term exposure of SH-SY5Y cells to morphine: a whole cell proteomic analysis

Neasta, Jérémie; Uttenweiler-Joseph, Sandrine; Chaoui, Karima; Monsarrat, Bernard; Meunier, Jean-Claude; Moulédous, Lionel

doi:10.1186/1477-5956-4-23

Proteome Science

Table 3 Proteins whose abundance in SH-SY5Y cells is regulated after 72 h of morphine treatment

From: Effect of long-term exposure of SH-SY5Y cells to morphine: a whole cell proteomic analysis

Spot #	Protein	Accession # (a)	MW (Da)	Variation (% of control) (b)	MALDI peptides	% coverage	MS/MS peptides (c)
4	Radixin	P35241	68564	50 ± 17			4
6	no identification			48 ± 23
8	Heat shock cognate 71 kDa protein	P11142	70898	172 ± 48	11	23
10	Lamin A/C	P02545	74139	78 ± 15	22	30
12	T-complex protein 1, zeta subunit	P40227	57893	57 ± 17	15	29
13	Dihydropyrimidinase-related	Q14195	61963	57 ± 21	13	40
	protein 3
17	Septin-11	Q9NVA2	49267	110 ± 3	13	37
23	Rab GDP dissociation inhibitor beta	P50395	50663	179 ± 61	11	34
24	Rab GDP dissociation inhibitor beta Mitochondrial-processing peptidase beta subunit	P50395 O75439	50663 55072	114 ± 6	14 12	36 25
25	Proliferation-associated protein 2G4	Q9UQ80	43787	54 ± 20	15	44
26	Elongation factor 1-gamma	P26641	49988	89 ± 5	16	36
28	Adenylosuccinate synthetase 2	P30520	50097	73 ± 16			5
29	Elongation factor 1-gamma tRNA-nucleotidyltransferase 1	P26641 Q96Q11	49988 50340	51 ± 27	11 9	24 19
31	Ornithine aminotransferase.	P04181	48535	84 ± 3	14	37
34	Septin-2	Q15019	41487	72 ± 15	12	36
39	Ubiquitin-like 1 activating enzyme E1A	Q9UBE0	38450	45 ± 14	11	35
40	Guanine nucleotide binding protein, alpha inhibiting activity polypeptide 2	Q96C71	40493	65 ± 8	9	37
43	3-mercaptopyruvate sulfurtransferase	P25325	33047	69 ± 9			6
44	Malate dehydrogenase, cytoplasmic	P40925	36295	73 ± 16	11	32
45	Alpha-soluble NSF attachment protein	P54920	33247	45 ± 12	8	45
47	Nuclear protein Hcc-1	P82979	23540	77 ± 9	9	41
48	no identification			49 ± 32
49	Guanine nucleotide binding protein beta subunit 2-like 1	P63244	35077	74 ± 9	15	69
50	Proteasome subunit alpha type 3	P25788	28302	73 ± 13	8	46
55	Proteasome subunit beta type 6	P28072	25358	143 ± 11	7	23

^aSwiss-Prot primary accession number
^bMean ± S.D., control refers to untreated cells.
^cOnly peptides with a statistically significant Mascot score (p < 0.05) were considered

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ISSN: 1477-5956

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