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Figure 3 | Proteome Science

Figure 3

From: A systems approach to clinical oncology: Focus on breast cancer

Figure 3

Analysis of serum proteins using ProteinChip arrays and SELDI-TOF/MS. A. Crude serum sample is placed (and processed) on a ProteinChip array which contains chemically (cationic, anionic, hydrophobic, hydrophilic, etc.) or biologically treated surfaces for specific interaction with proteins of interest. Proteins, thus, bind to chemical or biological "docking sites" on the ProteinChip surface. Non-binding proteins, salts, and other contaminants are washed away, eliminating sample "noise". B. Retained proteins are "eluted" from the ProteinChip array by Surface-Enhanced Laser Desorption/Ionization (SELDI). Ionized proteins are detected and their mass accurately determined by Time-of-Flight Mass Spectrometry (TOF/MS).

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