Skip to main content

Table 1 List of proteins on the 2-DE map of Halobacterium NRC-1, identified byMALDI-TOF and LC-MS/MS

From: Proteomic analysis of acidic chaperones, and stress proteins in extreme halophile Halobacterium NRC-1: a comparative proteomic approach to study heat shock response

No.

ORF No.

Protein Name

MS/MASCOT Score

Mowse Score

% Coverage

Annotation

Putative kDa/pI

Calculated kDa/pI

1

2338

polA2*

15

39

13

DNA polymerase

150/4.38

148.9/4.39

2

2096

cctB

668

 

33

Thermosome β

70/4.12

69/4.1

3

2381

uvrC*

40

51

18

Excision nuclease chain C

63.7/4.8

63/4.89

4

6363

Orc4/CDC6

82

 

30

Orc4/CDC6

58/4.51

58/4.52

5

715

thiC

57

 

28

Thiamine biosynthesis protein

51/4.15

51.5/4.15

6

1601

gcvP2*

20

47

20

Glycine Dehydrogenase II

50/4.43

51/4.4

7

1356

fumC

378

 

27

Fumarate hydratase

50/4.4

50/4.38

8

2649

eef1A

158

 

18

Translation elongation factor α subunit

46/4.32

46.2/4.3

9

2606

thiD

83

 

21

Thiamine biosynthesis protein

45/4.2

47/4.17

10

95

gapB

220

 

13

Glyceraldehyde 3-PDH

35/4.26

35.5/4.2

11

903

yufN*

20

47

8

ABC transporter

33/4.02

33.2/4.02

12

683

vng0683C*

73

63

14

Hypothetical

28/4.69

28.9/4.6

13

2331

vng0309C*

56

46

12

Hypothetical

25/4.48

26.9/4.31

14

1027

tpiA

50

 

13

Triosephosphate isomerase

21/3.97

21.3/4.01

15

2443

dpsA

60

 

19

Stress induced DNA binding protein

20/4.14

20.4/4.16

16

890

imD2

196

 

25

Inosine-5'-monophosphate dehydrogenase

20/4.76

20.1/4.8

17

1914

ppiA*

20

45

11

Peptidyl proryl isomerase

20/4.02

21/4.1

18

633

purE

72

 

20

Phosphoribosylamino subunit

21/4

21.7/4.07

19

1180

msrA

71

 

38

Peptide methionine sulfoxide red.

19.4/4.2

21.1/4.18

20

1607

cheC2

76

 

12

Chemotaxis protein

21/4.23

21.7/4.2

21

2118

pyrE2*

25

51

22

Orotatephosphoribosyl transferase

18/4.11

19.2/4.13

22

626

maoC2*

27

52

22

Acyl Dehydratase

16.8/4.15

16.9/4.2

23

1807

vng1807H

55

 

38

Hypothetical

15/4.29

16/4.32

24

6254

vng6254*

21

42

12

Conserved Hypothetical

11.4/3.85

12.2/3.9

25

1137

rpl18E*

22

44

17

Ribosome assembly

12.5/5.6

12.1/5.62

26

1157

rphs6*

30

47

12

Ribosome assembly

12.7/3.79

12.5/3.81

27

2467

rpl31E

110

 

71

Ribosome assembly

10/4.52

10.2/4.4

28

1922

trh5*

32

54

26

Lrp like regulator

8.0/4.24

8.4/4.11

29

1300

vng1300H*

49

71

91

Hypothetical

6.5/3.91

8.0/4.0

30

2047

rps27aE*

30

49

10

Ribosome assembly

6/3.87

8.2/3.86

  1. *Proteins identified by MALDI-TOF