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Table 1 List of proteins on the 2-DE map of Halobacterium NRC-1, identified byMALDI-TOF and LC-MS/MS

From: Proteomic analysis of acidic chaperones, and stress proteins in extreme halophile Halobacterium NRC-1: a comparative proteomic approach to study heat shock response

No. ORF No. Protein Name MS/MASCOT Score Mowse Score % Coverage Annotation Putative kDa/pI Calculated kDa/pI
1 2338 polA2* 15 39 13 DNA polymerase 150/4.38 148.9/4.39
2 2096 cctB 668   33 Thermosome β 70/4.12 69/4.1
3 2381 uvrC* 40 51 18 Excision nuclease chain C 63.7/4.8 63/4.89
4 6363 Orc4/CDC6 82   30 Orc4/CDC6 58/4.51 58/4.52
5 715 thiC 57   28 Thiamine biosynthesis protein 51/4.15 51.5/4.15
6 1601 gcvP2* 20 47 20 Glycine Dehydrogenase II 50/4.43 51/4.4
7 1356 fumC 378   27 Fumarate hydratase 50/4.4 50/4.38
8 2649 eef1A 158   18 Translation elongation factor α subunit 46/4.32 46.2/4.3
9 2606 thiD 83   21 Thiamine biosynthesis protein 45/4.2 47/4.17
10 95 gapB 220   13 Glyceraldehyde 3-PDH 35/4.26 35.5/4.2
11 903 yufN* 20 47 8 ABC transporter 33/4.02 33.2/4.02
12 683 vng0683C* 73 63 14 Hypothetical 28/4.69 28.9/4.6
13 2331 vng0309C* 56 46 12 Hypothetical 25/4.48 26.9/4.31
14 1027 tpiA 50   13 Triosephosphate isomerase 21/3.97 21.3/4.01
15 2443 dpsA 60   19 Stress induced DNA binding protein 20/4.14 20.4/4.16
16 890 imD2 196   25 Inosine-5'-monophosphate dehydrogenase 20/4.76 20.1/4.8
17 1914 ppiA* 20 45 11 Peptidyl proryl isomerase 20/4.02 21/4.1
18 633 purE 72   20 Phosphoribosylamino subunit 21/4 21.7/4.07
19 1180 msrA 71   38 Peptide methionine sulfoxide red. 19.4/4.2 21.1/4.18
20 1607 cheC2 76   12 Chemotaxis protein 21/4.23 21.7/4.2
21 2118 pyrE2* 25 51 22 Orotatephosphoribosyl transferase 18/4.11 19.2/4.13
22 626 maoC2* 27 52 22 Acyl Dehydratase 16.8/4.15 16.9/4.2
23 1807 vng1807H 55   38 Hypothetical 15/4.29 16/4.32
24 6254 vng6254* 21 42 12 Conserved Hypothetical 11.4/3.85 12.2/3.9
25 1137 rpl18E* 22 44 17 Ribosome assembly 12.5/5.6 12.1/5.62
26 1157 rphs6* 30 47 12 Ribosome assembly 12.7/3.79 12.5/3.81
27 2467 rpl31E 110   71 Ribosome assembly 10/4.52 10.2/4.4
28 1922 trh5* 32 54 26 Lrp like regulator 8.0/4.24 8.4/4.11
29 1300 vng1300H* 49 71 91 Hypothetical 6.5/3.91 8.0/4.0
30 2047 rps27aE* 30 49 10 Ribosome assembly 6/3.87 8.2/3.86
  1. *Proteins identified by MALDI-TOF
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