Representative SELDI-TOF MS spectra of CSF in denaturing buffer on CM10, Q10, H50 and IMAC30 arrays. a) CSF was diluted 1:1 in denaturing buffer 9.5 M urea, 2% CHAPS, 50 mM Tris-HCl, pH 9.0. Denatured CSF samples were diluted 1:4 in appropriate binding buffer and processed on: CM10 with 100 mM ammonium acetate pH 4.0; Q10 with 100 mM Tris-HCl pH 9.0; H50 with 10% AcN, 01% TFA; and IMAC30 with 100 mM Na phosphate, 0.5 M NaCl pH 7.0 and 100 mM Cu sulphate activation. ProteinChip arrays were prepared with SPA. b) Enlargement of the shaded spectra region showing peaks between m/z range of 4–11 kDa.