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Figure 6 | Proteome Science

Figure 6

From: Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid

Figure 6

Assessment of systematic variability across bioprocessor plates using principle component analysis. Variability was evaluated across three bioprocessor plates processed 4 and 24 hours apart. Spectra were obtained from pooled CSF sample randomly placed on a single spot across each of twelve different CM10 chips on one bioprocessor plate. The same was repeated for the other plates 4 and 24 hr later. Following baseline subtraction, normalization and spectra alignment, 45 peaks which appeared in all spectra were used for PCA analysis. The PCA results were color coded for the three bioprocessor plates: blue, 0 hr; red, 4 hr; black, 24 h.

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ISSN: 1477-5956