Proteome Science

Table 1 Comparison of peak count between all tested conditions using SPA as matrix.

From: Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid

		Native		9 M urea, 2% CHAPS		9 M urea, 2% CHAPS, 10 mM DTT
		Peak count	Average	Peak count	Average	Peak count	Average
CM10	w/o Triton X-100	103	103	121	117	102	100
		104		112		90
		101		119		99
	Triton X-100	100	102	105	104	95	98
		104		105		102
		101		102		90
Q10	w/o Triton X-100	101	102	109	109	103	100
		103		108		97
		102		110		100
	Triton X-100	89	90	105	106	104	104
		90		105		104
		91		108		103
H50	10% AcN, 01% TFA	104	101	103	105	98	102
		99		103		109
		100		109		101
	PBS	76	79	74	72	77	76
		82		74		74
		78		70		77
IMAC30	Copper	102	99	100	113	97	103
		95		110		106
		97		131		106
	Nickel	107	105	88	92	84	81
		102		90		70
		103		100		90

Peaks with a signal-to-noise of 3 or greater were assigned between the m/z range of 2.5–20 kDa. 5 μL of pooled CSF prepared under native, denaturing, and denaturing/reducing buffer was applied in triplicate to each condition tested on CM10, Q10, H50, and IMAC30 arrays.

Back to article page

ISSN: 1477-5956

Contact us

General enquiries: journalsubmissions@springernature.com