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Table 1 Comparison of peak count between all tested conditions using SPA as matrix.

From: Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid

   Native 9 M urea, 2% CHAPS 9 M urea, 2% CHAPS, 10 mM DTT
   Peak count Average Peak count Average Peak count Average
CM10 w/o Triton X-100 103 103 121 117 102 100
   104   112   90  
   101   119   99  
  Triton X-100 100 102 105 104 95 98
   104   105   102  
   101   102   90  
Q10 w/o Triton X-100 101 102 109 109 103 100
   103   108   97  
   102   110   100  
  Triton X-100 89 90 105 106 104 104
   90   105   104  
   91   108   103  
H50 10% AcN, 01% TFA 104 101 103 105 98 102
   99   103   109  
   100   109   101  
  PBS 76 79 74 72 77 76
   82   74   74  
   78   70   77  
IMAC30 Copper 102 99 100 113 97 103
   95   110   106  
   97   131   106  
  Nickel 107 105 88 92 84 81
   102   90   70  
   103   100   90  
  1. Peaks with a signal-to-noise of 3 or greater were assigned between the m/z range of 2.5–20 kDa. 5 μL of pooled CSF prepared under native, denaturing, and denaturing/reducing buffer was applied in triplicate to each condition tested on CM10, Q10, H50, and IMAC30 arrays.