Table 1 D. melanogaster accessory gland proteins identified by proteomics. AG proteins with a secretory signal peptide identified by either MALDI-MS or LC-MS/MS of tryptic fragments of AG protein spots excised from 2D gels. Data presented in parenthesis is derived from MALDI-MS analysis
CG number | Protein name | Homology | Predicted function | Number of peptides matched | % coverage | Estimated protein Mr (kDa) | Theoretical Mr (kDa) |
|---|---|---|---|---|---|---|---|
CG2852 | peptidyl-prolyl cis-trans isomerase | protein folding | 11(8) | 57 (46) | 20 | 22 | |
CG2918 | heat shock protein 70 kDA | chaperone | 14 | 17 | 106 | 103 | |
CG4147 | Hsc-70-3 | heat shock protein | chaperone | (15) | (26) | 71 | 72 |
CG4847 | cathepsin K | peptidase | 3 (9) | 8 (39) | 42 | 44 | |
CG5520 | Gp93 | histidine kinase-like ATPase | chaperone | 2 | 3 | 92 | 90 |
CG6461 | Ggt-1 | γ-glutamyl transpeptidase | peptidase | 2 | 5 | 24 | 63 |
CG6988 | Pdi | protein disulfide isomerase | protein folding | 8 | 23 | 62 | 56 |
CG7157 | Acp36DE | hormone | (3) | (3) | 102 | 102 | |
CG9334 | Spn3 | serpin | peptidase inhibitor | (14) | (45) | 45 | 42 |
CG9429 | calreticulin | chaperone | chaperone | (8) | (21) | 53 | 47 |
CG9847 | Fkbp13 | peptidyl-prolyl cis-trans isomerase | protein folding | 2 | 12 | 26 | 24 |
CG17575 | CRISP | (6) | (25) | 33 | 32 | ||
CG31872 | triacylglycerol lipase | lipid metabolism | 6 (4) | 7 (4) | 118 | 119 |