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Table 2 Peptidase activity of D. melanogaster accessory glands. Results are expressed as the mean of three experiments ± s.e.m

From: Proteomic identification of Drosophila melanogaster male accessory gland proteins, including a pro-cathepsin and a soluble γ-glutamyl transpeptidase

Enzyme activity

Rate of hydrolysis (pmol of 7-AMC released/h/AG)

Cysteine peptidase

 

   pre-activation

0.7 ± 0.1

   post-activation

4.5 ± 1.3

   post-activation + 0.1 μM E64

0.18 ± 0.01

   post-activation + 0.1 μM K11777

0.25 ± 0.03