Figure 2
From: Proteomic analysis of tyrosine phosphorylation during human liver transplantation
Identification of tyrosine phosphorylated proteins upon I/R. A. Schematic representation of the approach used for tyrosine phosphorylated proteins identification. PY matrix: anti-phosphotyrosine antibodies coupled to agarose beads. B. Representative SDS-PAGE experiment after tyrosine immunoprecipitation (Ip PY) of 60 min ischemia (I60) and 60 min reperfusion (R60) protein extracts. The band marked by an arrow has been further identified as the SH2/SH3 adaptor Nck-1. N = 1 on 2 independent pools of 3 liver biopsy protein extracts. C. Pie-chart representation of the total number of proteins identified with a significant Mascot score by at least one unique peptide for the 60 min ischemia (I60, left panel) and the 60 min reperfusion (R60, right panel). The proteins were classified in functional families according to their GO (Gene Ontology) annotation.