Figure 2

2-DE gel images of SYPRO Ruby-stained secretome proteins of toxigenic, non-encapsulated B. anthracis strain RA3R (pXO1⁺/pXO2^-) from pH 4 to 7 at 6 hr (A), 10 hr (B) and 16 hr (C) after inoculation under conditions that simulate those found inside the host (induced), and (D) Western blot analysis of immunogenic extracellular proteins at 16 hr using sera from patients infected with cutaneous anthrax. Spot numbers refer to additional file 1.