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Table 1 Classification of proteins identified from fractions enriched for plasma membrane and secretory vesicles.

From: Proteomic analysis of plasma membrane and secretory vesicles from human neutrophils

Plasma membrane vesicles

Secretory vesicles

Adhesion

Integrin alpha-M (CD11b)

Integrin beta-2 (CD18 antigen)

Integrin alpha-IIb

Intercellular adhesion molecule-3 (ICAM-3)

Phagocytic glycoprotein I (CD44 antigen)

Integrin alpha-M (CD11b)

Integrin beta-2 (CD18 antigen)

ADP-ribosyl cyclase 2 (CD157 antigen)

Erythrocyte band 7 integral membrane protein (Stomatin)

Cytoskeletal

Beta-actin (ACTB)

Alpha-actinin 1

Alpha-actinin 4

Cofilin, non-muscle form

Coronin-1A

Moesin

Myosin light polypeptide 6

Myosin regulatory light chain 2, nonsarcomeric

Tropomyosin alpha 3 chain

Tropomyosin beta chain

Beta-actin (ACTB)

Myosin-9

Tubulin alpha-ubiquitous chain

Signal transduction

Guanine nucleotide-binding protein G(i), alpha-2 subunit

Tyrosine protein kinase Lyn *

Flotillin*

B-cell receptor-associated protein 31

Chloride intracellular channel protein 1

Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1

HLA class I histocompatibility antigen, A-26 alpha chain

Interferon-induced transmembrane protein 1

Ras-related protein Rap-1A

Ras-related protein Rab-5A

Ras-related protein Rap-1b

Ras-related protein Rab-27B

Synaptosomal-associated protein 23

Guanine nucleotide-binding protein G(i), alpha-2 subunit

Tyrosine protein kinase Lyn *

Flotillin*

Adipocyte plasma membrane-associated protein

C5a anaphylatoxin chemotactic receptor

Dysferlin

Leukocyte surface antigen CD47

5-lipoxygenase activating protein (FLAP)

Solute carrier family 2, facilitated glucose transporter, member 3

NADPH oxidase

Cytochrome b-245 heavy chain (gp91phox )*

Cytochrome b-245 heavy chain (gp91phox)

Differentiation

 

Myeloid-associated differentiation marker (SB135)

Leukocyte common antigen (CD45 antigen)

Serum protein

Serum albumin

Serum albumin

Alpha-1-antitrypsin

Ig gamma-1 chain C region

Mitochondrial/Microsomal

 

ATP synthase alpha chain, mitochondrial

ATP synthase beta chain, mitochondrial

Citrate synthase, mitochondrial

Cytochrome P450 4F2

60 kDa heat shock protein, mitochondrial (Hsp60)

Isocitrate dehydrogenase [NADP], mitochondrial

Malate dehydrogenase, mitochondrial

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Sulfide: quinone oxidoreductase, mitochondrial

Trifunctional enzyme beta subunit, mitochondrial

Vacuolar ATP synthase subunit d

Metabolic

 

Aldehyde dehydrogenase 3B2

Dehydrogenase/reductase SDR family member 7

Tyrosine-protein phosphatase non-receptor type substrate 1

Granule

Lactoferrin

Matrix metalloproteinase 9

Myeloperoxidase

Cathepsin G

Azurocidin

Lactoferrin

Matrix metalloproteinase 9

Myeloperoxidase

Aminopeptidase N (CD13 antigen)

Eosinophil peroxidase

Cytosolic

14-3-3 protein zeta/delta

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit

Unknown

Actin-related protein 2

Golgi-associated plant pathogenesis related protein 1

Protein FAM49B

Tetratricopeptide repeat protein 10

ERO1-like protein alpha

Pantophysin

  1. * Proteins identified only by immunoblotting are listed in italics. All other proteins identified by mass spectrometry.