Figure 5

Western blot validation of selected proteins identified by iTRAQ and/or 2DE analysis. Equal amounts, 25 μg, of protein extracts from TOV-112D and TOV-81D cell lines were loaded onto a 12 % SDS-PAGE and processed for Western blotting with the indicated antibodies. Protein expression differences were quantified using a Chemilmager 4000 imaging system and AlphaEase software 3.3 (Alpha Innotech Corporation). The data are expressed as relative integrated density value (IDV). Each point represents the mean +/- SE from three independent experiments. Data were analysed by Student's unpaired t test. Representative blots for each analysis are depicted.