Comparative proteome analysis of human epithelial ovarian cancer

Gagné, Jean-Philippe; Éthier, Chantal; Gagné, Pierre; Mercier, Geneviève; Bonicalzi, Marie-Ève; Mes-Masson, Anne-Marie; Droit, Arnaud; Winstall, Eric; Isabelle, Maxim; Poirier, Guy G

doi:10.1186/1477-5956-5-16

Proteome Science

Table 1 iTRAQ analysis of differentially expressed proteins between human epithelial ovarian cancer cell lines TOV-112D and TOV-81D†.

From: Comparative proteome analysis of human epithelial ovarian cancer

Accession	Entrez Gene	GO	Gene	SwissProt	Description	Number of unique peptides	Protein ratio (112D/81D)	%RSD	Validation
IPI00008557	10642	RNA metabolism/protein biosynthesis	IGF2BP1	Q9NZI8	Insulin-like growth factor 2 mRNA-binding protein 1	2	11,9	81
IPI00005996	8091	Transcription	HMGA2	P52926	High mobility group protein HMGI-C	2	8,1	100
IPI00218914	216	Response to stress	ALDH1A1	P00352	Aldehyde dehydrogenase 1A1	6	5,8	43	2D
IPI00021033	1281	Cell adhesion	COL3A1	P02461	Collagen alpha-1(III) chain precursor	4	5,0	79	2D
IPI00014424	1917	Protein biosynthesis	EEF1A2	Q05639	Elongation factor 1-alpha 2	3	4,2	80	2D
IPI00028376	1678	Transport/protein metabolism	TIMM8A	O60220	Mitochondrial import inner membrane translocase subunit Tim8A	2	4,2	14
IPI00030131	7112	Cytoskeleton organizationanisation	TMPO	P42167	Lamina-associated polypeptide 2, isoforms beta/gamma	3	3,8	21
IPI00329745	10128	RNA metabolism	LRPPRC	P42704	130 kDa leucine-rich protein	3	3,8	30
IPI00301189	51477	Lipid biosynthesis	ISYNA1	Q9NPH2	Myo-inositol 1-phosphate synthase A1	2	3,7	23
IPI00165467	10643	RNA metabolism/protein biosynthesis	IGF2BP3	O00425	Insulin-like growth factor 2 mRNA-binding protein 3	3	3,3	50
IPI00292387	9221	RNA metabolism	NOLC1	Q14978	Nucleolar phosphoprotein p130	2	3,3	22
IPI00218493	3251	Puridine salvage	HPRT1	P00492	Hypoxanthine-guanine phosphoribosyltransferase	2	3,1	15
IPI00218918	301	Membranes binding/cell motility	ANXA1	P04083	Annexin A1	7	3,0	41	2D&WB
IPI00216044	22913	RNA metabolism	RALY	Q9UKM9	RNA-binding protein Raly	2	3,0	18
IPI00000105	9961	Response to stress	MVP	Q14764	Major vault protein	3	0,40	87
IPI00455315	302	Membranes binding/cell motility	ANXA2	P07355	Annexin A2	3	0,40	33
IPI00477536	2317	Cell motility/Cytoskeleton organization/signal transduction	FLNB	Q60FE7	Filamin B	2	0,38	47
IPI00333541	2316	Cell motility/Cytoskeleton organization/signal transduction	FLNA	P21333	Filamin-A	10	0,36	46
IPI00296099	7057	Cell motility/cell adhesion	THBS1	P07996	Thrombospondin-1 precursor	2	0,35	67
IPI00013808	81	Cell motility/cell adhesion	ACTN4	O43707	Alpha-actinin-4	2	0,33	22
IPI00029111	1809	Pyrimidine metabolism	DPYSL3	Q6DEN2	Dihydropyrimidinase-like 3 protein (DPYSL3 protein)	4	0,30	85	2D
IPI00554788	3875	Cytoskeleton organizationanisation	KRT18	P05783	Keratin-18	2	0,29	19
IPI00306604	3678	Cell adhesion	ITGA5	P08648	Integrin alpha-5 precursor	2	0,29	45
IPI00163187	6624	Cytoskeleton organization/cell motility	FSCN1	Q16658	Fascin	2	0,24	18	2D
IPI00031008	3371	Cell adhesion	TNC	P24821	Tenascin-C	2	0,23	71
IPI00182373	8974	Collagen biosynthesis	P4HA2	O15460	Prolyl 4-hydroxylase subunit alpha-2 precursor	5	0,22	22	2D
IPI00472165	5352	Collagen biosynthesis	PLOD2	O00469	Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 precursor;	3	0,21	55	2D
IPI00550363	8407	Cytoskeleton organization/cell motility	TAGLN2	P37802	Transgelin-2	2	0,20	11	2D&WB
IPI00216138	6876	Cytoskeleton organization/cell motility	TAGLN	Q01995	Transgelin	5	0,20	58
IPI00008494	3383	Cell adhesion	ICAM1	P05362	Intercellular adhesion molecule 1 precursor (ICAM1)	4	0,17	35
IPI00216135	7168	Cell motility/cell adhesion	TPM1	P09493	Tropomyosin alpha-1 chain	2	0,16	38	2D
IPI00007118	5054	Cell adhesion/motility/urokinase regulation	SERPINE1	P05121	Plasminogen activator inhibitor 1 precursor (PAI-1)	2	0,15	27	2D
IPI00414283	2335	Cell motility/cell adhesion	FN1	P02751	Fibronectin precursor	2	0,11	8
IPI00026663	220	Response to stress	ALDH1A3	P47895	Aldehyde dehydrogenase 1A3	3	0,11	39	2D
IPI00442073	1465	Cell motility	CSRP1	P21291	Cysteine and glycine-rich protein 1	3	0,08	255
IPI00297646	1277	Cell adhesion	COL1A1	P02452	Collagen alpha-1(I) chain precursor	5	0,08	50
IPI00178352	2318	Cell motility/Cytoskeleton organization/signal transduction	FLNC	Q14315	Filamin-C	3	0,07	39

†Selected protein expression ratios were confirmed by western blot (WB) analysis. Differentially expressed proteins also identified by comparative two-dimensional electrophoresis are indicated (2D). %RSD = (standard deviation/mean) × 100

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ISSN: 1477-5956

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