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Figure 1 | Proteome Science

Figure 1

From: Quantifying raft proteins in neonatal mouse brain by 'tube-gel' protein digestion label-free shotgun proteomics

Figure 1

Biochemical characterization of sucrose density gradient fractions of neonatal mouse brain. Panel A shows the distributions of protein and sucrose density in membrane fractions from sucrose gradients of neonatal brain. Panel B shows the light-scattering properties of each fraction by absorbance at 620 nm. The buoyant low density fractions 2~4 showed the greatest light-scattering properties at 620 nm, consistent with a high content of lipids. Panel C shows the distribution of sterols (cholesterol, desmosterol, lathosterol, and 7-dehydrodesmosterol (7DHD)) in each fraction from sucrose gradients of neonatal mouse brain and Panel D shows total sphingomyelin (SM) and ceramide (Cer).

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Proteome Science

ISSN: 1477-5956