Table 2 Non-PM proteins identified in the lipid rafts of neonatal mouse brain
Acc. No. | Protein name | Pep a | % Cov b | MW (KDa) | Loc.c | Spectral count d | SD e |
|---|---|---|---|---|---|---|---|
P69893 | Tubulin beta-1 chain | 12 | 38.60 | 49.67 | cyto | 262.0 | 14.0 |
P68361 | Tubulin alpha-1 chain | 9 | 30.44 | 50.15 | cyto | 175.0 | 20.0 |
Q71FK5 | Actin, cytoplasmic 1 (Beta-actin) | 5 | 21.39 | 41.74 | cyto | 102.5 | 4.5 |
Q03265 | ATP synthase alpha chain | 10 | 26.63 | 59.75 | mc | 63.0 | 4.0 |
P56480 | ATP synthase beta chain | 9 | 20.83 | 56.30 | mc | 51.5 | 5.5 |
P04104 | Keratin, type II cytoskeletal 1 | 3 | 5.59 | 65.1 | cyto | 37.0 | 4.0 |
P62629 | Elongation factor 1-alpha 1 | 3 | 9.33 | 50.11 | cyto | 36.0 | 5.0 |
P19378 | Heat shock cognate 71 kDa protein | 4 | 10.39 | 70.8 | cyto | 33.0 | 1.0 |
Q922U2 | Keratin, type II cytoskeletal 5 | 2 | 4.15 | 61.8 | cyto | 26.5 | 6.5 |
P14873 | Microtubule-associated protein 1B (MAP 1B) | 7 | 4.34 | 270.41 | cyto | 25.5 | 3.5 |
Q6IFZ6 | Keratin, type II cytoskeletal 1b | 2 | 4.20 | 61.4 | cyto | 25.5 | 3.5 |
P97427 | Dihydropyrimidinase-related protein 1 | 4 | 8.93 | 62.17 | cyto | 25.0 | 0.0 |
Q62188 | Dihydropyrimidinase-related protein 3 | 7 | 20.39 | 61.94 | cyto | 21.5 | 0.5 |
P14733 | Lamin-B1 | 5 | 12.80 | 66.66 | nuc | 19.0 | 3.0 |
P68372 | Tubulin beta-2C chain | 2 | 6.76 | 49.83 | cyto | 18.5 | 1.5 |
Q6IG00 | Keratin, type II cytoskeletal 4 | 2 | 1.68 | 57.7 | cyto | 18.0 | 1.0 |
Q60932 | Voltage-dependent anion-selective channel protein 1 | 7 | 38.31 | 32.35 | mc | 16.5 | 3.5 |
P48962 | ADP/ATP translocase 1 | 3 | 11.15 | 32.77 | mc | 15.0 | 2.0 |
Q922F4 | Tubulin beta-6 chain | 2 | 3.81 | 50.09 | cyto | 14.5 | 0.5 |
Q04447 | Creatine kinase B-type | 3 | 12.63 | 42.71 | cyto | 13.5 | 2.5 |
P67778 | Prohibitin | 4 | 21.03 | 29.82 | mc | 12.5 | 1.5 |
Q10758 | Keratin, type II cytoskeletal 8 | 2 | 3.33 | 53.9 | cyto | 12.5 | 0.5 |
Q61696 | Heat shock 70 kDa protein 1A | 2 | 5.78 | 70.08 | cyto | 12.0 | 1.0 |
O08553 | Dihydropyrimidinase-related protein 2 | 5 | 13.84 | 62.17 | cyto | 11.5 | 2.5 |
P46633 | Heat shock protein HSP 90-alpha (HSP 86) | 2 | 3.56 | 84.72 | cyto | 10.5 | 2.5 |
P63101 | 14-3-3 protein zeta/delta | 2 | 12.30 | 27.77 | cyto | 10.5 | 0.5 |
Q60930 | Voltage-dependent anion-selective channel protein 2 | 3 | 14.29 | 31.73 | mc | 10.0 | 0.0 |
Q9ERD7 | Tubulin beta-3 chain | 2 | 9.35 | 50.42 | cyto | 10.0 | 1.0 |
P50672 | Cytochrome c oxidase subunit 2 | 2 | 11.50 | 25.82 | mc | 9.0 | 0.0 |
Q9DCT2 | NADH-ubiquinone oxidoreductase 30 kDa subunit | 2 | 6.35 | 34.00 | mc | 8.5 | 0.5 |
P18760 | Cofilin-1 (Cofilin, non-muscle isoform) | 2 | 15.24 | 18.43 | nuc | 8.5 | 1.5 |
P07823 | 78 kDa glucose-regulated protein | 5 | 11.18 | 72.38 | er | 8.5 | 1.5 |
P11497 | Acetyl-CoA carboxylase 1 | 1 | 4.24 | 37.62 | cyto | 8.0 | 1.0 |
Q60931 | Voltage-dependent anion-selective channel protein 3 | 3 | 13.83 | 30.75 | mc | 7.5 | 0.5 |
P09445 | Elongation factor 2 | 2 | 2.92 | 95.27 | cyto | 7.5 | 0.5 |
P62977 | Ubiquitin | 1 | 21.33 | 8.57 | cyto | 7.0 | 1.0 |
P19783 | Cytochrome c oxidase subunit IV isoform 1 | 1 | 7.14 | 19.53 | mc | 7.0 | 2.0 |
P14152 | Malate dehydrogenase | 1 | 3.61 | 36.35 | mc | 6.5 | 0.5 |
P11499 | Heat shock protein HSP 90-beta | 2 | 3.88 | 83.20 | cyto | 6.5 | 0.5 |
P12787 | Cytochrome c oxidase polypeptide Va | 1 | 10.42 | 16.03 | mc | 6.5 | 0.5 |
P31253 | Ubiquitin-activating enzyme E1 X | 2 | 7.35 | 50.99 | cyto | 6.0 | 3.0 |
P35564 | Calnexin | 2 | 4.92 | 67.28 | er | 6.0 | 1.0 |
Q91VD9 | NADH-ubiquinone oxidoreductase 75 kDa subunit | 2 | 3.99 | 79.75 | mc | 5.5 | 2.5 |
P56135 | ATP synthase f chain, mitochondrial | 2 | 26.74 | 10.21 | mc | 5.5 | 1.5 |
P51881 | ADP/ATP translocase 2 | 2 | 8.11 | 32.80 | mc | 5.5 | 0.5 |
Q8R429 | SR Ca(2+)-ATPase 1 | 2 | 3.12 | 109.43 | er | 5.0 | 1.0 |
Q9DB20 | ATP synthase O subunit | 2 | 9.91 | 23.36 | mc | 5.0 | 1.0 |
Q8R429 | Calcium pump 1 (SERCA1) | 2 | 3.12 | 109.43 | er | 5.0 | 1.0 |
Q91V61 | Sideroflexin-3 | 1 | 4.06 | 35.41 | mc | 4.5 | 0.5 |
P03995 | Glial fibrillary acidic protein, astrocyte (GFAP) | 1 | 2.56 | 49.92 | cyto | 4.5 | 1.5 |
Q9CQV8 | 14-3-3 protein beta/alpha | 1 | 5.43 | 21.22 | cyto | 4.5 | 2.5 |
P68368 | Tubulin alpha-4 chain | 2 | 3.11 | 50.14 | cyto | 3.5 | 0.5 |
P62962 | Profilin-1 (Profilin I) | 2 | 21.74 | 14.83 | cyto | 3.5 | 0.5 |
Q8QZT1 | Acetyl-CoA acetyltransferase | 2 | 7.09 | 44.82 | mc | 3.5 | 0.5 |
P62962 | Profilin-1 | 2 | 21.74 | 14.82 | cyto | 3.5 | 0.5 |
Q02053 | Ubiquitin-activating enzyme E1 1 | 3 | 4.82 | 117.81 | cyto | 3.0 | 1.0 |
P42932 | T-complex protein 1 subunit theta | 3 | 6.59 | 59.43 | cyto | 3.0 | 2.0 |
O35129 | Prohibitin-2 | 4 | 21.03 | 29.82 | mc | 3.0 | 1.0 |
P31324 | Prkar2b | 1 | 4.11 | 46.04 | cyto | 3.0 | 0.0 |
P20357 | Microtubule-associated protein 2 (MAP 2) | 2 | 1.20 | 198.98 | cyto | 3.0 | 0.0 |
P34926 | Microtubule-associated protein 1A (MAP 1A) | 2 | 2.36 | 299.53 | cyto | 3.0 | 0.0 |
P52480 | Pyruvate kinase isozyme M2 | 4 | 12.48 | 57.76 | mc | 3.0 | 2.0 |
P63209 | S-phase kinase-associated protein 1A | 1 | 9.32 | 18.53 | cyto | 3.0 | 1.0 |
P60879 | Synaptonemal complex protein 3 | 3 | 6.01 | 27.1 | nuc | 3.0 | 1.0 |
O88809 | Neuronal migration protein doublecortin | 1 | 3.56 | 40.61 | cyto | 2.5 | 0.5 |
P17156 | Heat shock-related 70 kDa protein 2 | 2 | 3.01 | 69.74 | cyto | 2.5 | 1.5 |
Q9EQF6 | Dihydropyrimidinase-related protein 5 | 1 | 3.20 | 61.52 | cyto | 2.5 | 0.5 |
Q8BH59 | Calcium-binding mitochondrial carrier protein Aralar1 | 2 | 4.59 | 74.57 | mc | 2.5 | 0.5 |
P48670 | Vimentin | 1 | 4.25 | 51.85 | cyto | 2.5 | 1.5 |
P80315 | T-complex protein 1 subunit delta | 1 | 2.98 | 57.94 | cyto | 2.0 | 0.0 |
P11984 | T-complex protein 1 subunit alpha A | 1 | 4.14 | 60.34 | cyto | 2.0 | 0.0 |
Q9JKK8 | Serine-protein kinase ATR | 1 | 2.36 | 84.26 | nuc | 2.0 | 0.0 |
Q04899 | Serine/threonine-protein kinase PCTAIRE-3 | 1 | 3.78 | 51.85 | nuc | 2.0 | 1.0 |
Q99PT1 | Rho GDP-dissociation inhibitor 1 (Rho GDI 1) | 1 | 7.88 | 23.41 | er | 2.0 | 0.0 |
Q61879 | Myosin-10 | 1 | 3.09 | 49.59 | cyto | 2.0 | 0.0 |
P24638 | Lysosomal acid phosphatase | 1 | 2.13 | 48.51 | lysosome | 2.0 | 1.0 |
O70251 | Elongation factor 1-beta | 2 | 12.56 | 24.56 | cyto | 2.0 | 0.0 |
Q9CPQ8 | ATP synthase g chain, mitochondrial | 1 | 18.63 | 11.43 | mc | 2.0 | 0.0 |
O35627 | Orphan nuclear receptor NR1I3 | 1 | 2.52 | 40.89 | nuc | 2.0 | 1.0 |
P53026 | 60S ribosomal protein L10a | 1 | 6.98 | 24.78 | nuc | 2.0 | 1.0 |
P97524 | Very-long-chain acyl-CoA synthetase | 1 | 2.58 | 70.69 | er | 1.5 | 0.5 |
Q01853 | Transitional endoplasmic reticulum ATPase | 2 | 3.11 | 89.18 | cyto | 1.5 | 0.5 |
Q99JR1 | Sideroflexin-1 | 1 | 5.63 | 35.52 | mc | 1.5 | 0.5 |
Q62627 | PRKC apoptosis WT1 regulator protein | 1 | 4.53 | 35.87 | nuc | 1.5 | 0.5 |
Q9DCS9 | NADH-ubiquinone oxidoreductase PDSW subunit | 1 | 10.92 | 20.89 | mc | 1.5 | 0.5 |
P08249 | Malate dehydrogenase | 2 | 9.79 | 35.60 | mc | 1.5 | 0.5 |
Q8BGU5 | Cyclin fold protein 1 | 1 | 5.00 | 39.39 | nuc | 1.5 | 0.5 |
Q8CEE6 | PAS-kinase (PASKIN) | 1 | 1.16 | 151.27 | cyto | 1.5 | 0.5 |
P35980 | 60S ribosomal protein L18 | 1 | 6.99 | 21.5 | nuc | 1.5 | 0.5 |