Increase in local protein concentration by field-inversion gel electrophoresis

Table 2 Selective comparison of densitometric results from 2D PAGE analyses under FIGE versus CFE conditions

Spot number	Protein name	Pulsed (FIGE)	Control (CFE)	Percent Increase in Intensity (%)
		Densitometric intensity^#
1	Endoplasmin precursor	14414	8502	69
2	Regucalcin	11453	11006	4
3	Fructose-1,6-bisphosphatase	15358	13699	12.1
4	Malate dehydrogenase, cytoplasmic	14771	12522	17.9
5	Regucalcin Isoform	11266	*	N.A.
6	Lactoylglutathione lyase	8531	4603	85.3
7	Cytochrome b5	12585	5569	126
8	Glutathione S-transferase kappa1	18944	3813	396.8
9	Fatty acid-binding protein, liver	26493	6228	325.3
A	Internal calibrator	33593	31998	5

^#Densitometric results are given as the average of the intensity per unit area. For similar spots under comparison, approximately the same area was obtained for the calculation of intensity. * denotes that the software failed to detect any differences with respect to backgroud noise. All results were corrected for background noise. Percent enhanced intensity is the percentage of increase in intensity using the intensity values of CFE as the denominator and the difference in intensity as the numerator. N.A., Not available.

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