Increase in local protein concentration by field-inversion gel electrophoresis

Table 3 Selective comparison of MS results of protein spots identified from 2D PAGE under FIGE versus CFE conditions

Spot number^#	Protein name	Mass (kDa)/pI	Pulsed (FIGE)		Control (CFE)
			Score MASCOT	% Coverage	Score MASCOT	% Coverage
1	Endoplasmin precursor	92.8/4.74	535	21	143	13
2	Regucalcin	33.9/5.15	253	20	180	23
3	Fructose-1,6-bisphosphatase	37.2/6.18	457	27	358	28
4	Malate dehydrogenase, cytoplasmic	36.5/6.16	288	21	86	5
5	Regucalcin Isoform	33.9/5.20	493	30	*	*
6	Lactoylglutathione lyase	20.9/5.25	94	17	*	*
7	Cytochrome b5	15.1/4.96	74	41	*	*
8	Glutathione S-transferase kappa1	^25.7/8.97	113	16	*	*
9	Fatty acid-binding protein, liver	14.3/8.59	367	25	*	*

^#Protein spots 1–4 (Control) and 1–9 (Pulsed) as shown in Figure 6a were selected for LC-MS-MS analysis. * denotes indiscernible spots in control condition but discernible in pulsed conditions.

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