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Table 3 Selective comparison of MS results of protein spots identified from 2D PAGE under FIGE versus CFE conditions

From: Increase in local protein concentration by field-inversion gel electrophoresis

Spot number# Protein name Mass (kDa)/pI Pulsed (FIGE) Control (CFE)
    Score MASCOT % Coverage Score MASCOT % Coverage
1 Endoplasmin precursor 92.8/4.74 535 21 143 13
2 Regucalcin 33.9/5.15 253 20 180 23
3 Fructose-1,6-bisphosphatase 37.2/6.18 457 27 358 28
4 Malate dehydrogenase, cytoplasmic 36.5/6.16 288 21 86 5
5 Regucalcin Isoform 33.9/5.20 493 30 * *
6 Lactoylglutathione lyase 20.9/5.25 94 17 * *
7 Cytochrome b5 15.1/4.96 74 41 * *
8 Glutathione S-transferase kappa1 25.7/8.97 113 16 * *
9 Fatty acid-binding protein, liver 14.3/8.59 367 25 * *
  1. #Protein spots 1–4 (Control) and 1–9 (Pulsed) as shown in Figure 6a were selected for LC-MS-MS analysis. * denotes indiscernible spots in control condition but discernible in pulsed conditions.