Figure 2
From: Age-related subproteomic analysis of mouse liver and kidney peroxisomes
Representatives 2-DE gels from peroxisome-enriched fractions were isolated from liver and kidney of M. musculus and separated by 2-DE with denaturing isoelectric focusing (IEF) on immobilized pH gradients in the first dimension between pH 3–10 non-linear (11 cm) and SDS-PAGE 12.5% in the second dimension. Gels were calibrated for molecular mass (in kDa) and pI (in pH units) by external pH and mass standards and stained by colloidal Coomassie. Differential expressed proteins are marked with numbers in A for liver and D for kidney. A. tissue: liver, age: 10 weeks; B. tissue: liver, age: 18 months; C. tissue: liver, age: 24 months; D. tissue: kidney, age: 10 weeks; E. tissue: kidney, age: 18 months; F. tissue: kidney, age: 24 months.