Figure 5

SDS-PAGE, immunoblotting from the different peroxisome-enriched fractions. A. The increase in protein expression in a one-dimensional gel was followed by loading the same amount of protein (20 μg each) of the peroxisome-enriched fraction from liver and kidney samples of 10 weeks old, 18 months old and 24 months (lane P) onto a 12.5% T polyacrylamide gel and stained by Coomassie blue. B. Immunoblot against AOX antibody.C. Fragment from the immunoblot against CAT antibody. C. Fragment from the immunoblot against PH antibody. E. Plot of the differential intensities from the immunoblot against anti-CAT, F. anti-PH and G. anti-AOX for the total and H. Values from the three subunits that immunoreact. In the x-axis, different group organized by tissue and age are represented and in the y-axis, differential band intensity in fold. The value from the band of the immunoblot was normalized against the 10 weeks old liver sample.