Figure 1

Effect of the use of a bioprocessor and sample denaturation on the urinary protein profile obtained with a SEND chip. 200 μl of 2-fold diluted non-denatured urine in 0.1% TFA was incubated using the bioprocessor (upper trace); 2 μl of non-denatured urine was directly applied to the spot without the use of a bioprocessor (middle trace); 2 μl of denatured urine directly applied to the spot without the use of a bioprocessor (lower trace). The same concentration was applied to all three conditions.